Green synthesized ZnO NPs as effective bacterial inhibitor against isolated MDRs and biofilm producing bacteria isolated from urinary tract infections

Abstract

Based on the importance of multi drug resistant and biofilm forming bacterial spread, the current research work aimed on synthesizing the nanoparticles of zinc oxide from the plant parts of Limonia acidissima. They were assayed for their antibacterial activities against biofilm forming urinary tract infected pathogens including Salmonella paratyphi, Shigella, Streptococcus, Staphylococcus and Klebsiella pneumonia which was confirmed by anti-microbial susceptibility test, 24-well polystyrene plate and modified tube test methods. The formulation of nanoparticles was confirmed by UV–Visible spectrophotometry. Fourier transform infrared spectroscopy revealed the formation of biomolecules that has great involvement in stabilizing the zinc oxide. The size as well as the shape with high resolution was confirmed with electron microscopical studies. It is very clear that zinc oxide nanoparticles have great antimicrobial effect because of its inhibition role against tested urinary tract bacteria by various invitro experiments. This provides a positive thinking on novel drug discovery, in which human health can be improved. Hence, this study provides a scientific support to the medicinal uses of zinc oxide nanoparticles for the treatment of microbial infections.

Keywords

• Nanoparticles
• Biomedical application
• Antibiotic susceptibility
• Minimum inhibition concentration