Serum AMH level predicts oocytes quality better than follicular fluid AMH level

Abstract

Background: Current in vitro fertilization (IVF) methods can't predict oocyte quality after oocyte retrieval. Anti-Mullerian hormones (AMH) in the follicular fluid are produced directly by granulosa cells therefore should be considered as parameter for predicting oocyte quality. The objective of this study is to develop a non-invasive method for predicting oocyte quality. Methods: This is a prognostic study with a cross sectional design, conducted from April 2012 to June 2013 at Yasmin IVF Clinic – Dr. Cipto Mangunkusumo General Hospital Jakarta. The subjects of this study were infertile couples underwent IVF. The measurement of serum AMH and estradiol level was performed at the beginning of IVF cycles, while follicular fluid AMH, were measured on the day of oocytes retrieval. Oocyte quality was measured using Xia morphology criteria. Result: We obtained 102 IVF patients with antagonist protocol. Serum and follicular fluid AMH, serum estradiol, number of mature oocytes, and oocytes morphological score were assessed. Serum AMH, follicular fluid AMH and serum estradiol were related to oocytes quality. Based on the multivariate analysis, we found that age and serum AMH level are the best two predictors for oocyte quality. Conclusion: Serum AMH, but not the follicular AMH, can be used to predict oocytes quality.

Keywords

• Serum and follicular AMH
• Oocytes quality
• In vitro fertilization