Nature Communications (Jul 2018)

Highly active enzymes by automated combinatorial backbone assembly and sequence design

  • Gideon Lapidoth,
  • Olga Khersonsky,
  • Rosalie Lipsh,
  • Orly Dym,
  • Shira Albeck,
  • Shelly Rogotner,
  • Sarel J. Fleishman

DOI
https://doi.org/10.1038/s41467-018-05205-5
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 9

Abstract

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Computationally designed enzymes often show lower activity or stability than their natural counterparts. Here, the authors present an evolution-inspired method for automated enzyme design, creating stable enzymes with accurate active site architectures and wild-type-like activities.