PLoS ONE (Jan 2014)

Inhibition of human and yeast 20S proteasome by analogues of trypsin inhibitor SFTI-1.

  • Dawid Dębowski,
  • Michał Pikuła,
  • Marta Lubos,
  • Paulina Langa,
  • Piotr Trzonkowski,
  • Adam Lesner,
  • Anna Łęgowska,
  • Krzysztof Rolka

DOI
https://doi.org/10.1371/journal.pone.0089465
Journal volume & issue
Vol. 9, no. 2
p. e89465

Abstract

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Starting from the primary structure of sunflower trypsin inhibitor SFTI-1, we designed novel non-covalent inhibitors of human and yeast 20S proteasomes. Peptides with Arg residue in P1 position and two basic amino acid residues (Lys or/and Arg) in P2' and P3' positions strongly inhibited chymotrypsin-like and caspase-like activities, while trypsin-like activity was poorly modified. We found that some SFTI-1 analogues up-regulated exclusively the chymotrypsin-like activity of latent yeast 20S proteasome.