Scientific Reports (2021-05-01)

Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment

  • Laura Sanchis-Artero,
  • Juan Francisco Martínez-Blanch,
  • Sergio Manresa-Vera,
  • Ernesto Cortés-Castell,
  • Marina Valls-Gandia,
  • Marisa Iborra,
  • Jose Maria Paredes-Arquiola,
  • Maia Boscá-Watts,
  • Jose Maria Huguet,
  • Rafael Gil-Borrás,
  • Josefa Rodríguez-Morales,
  • Xavier Cortés-Rizo

DOI
https://doi.org/10.1038/s41598-021-88823-2
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

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Abstract Intestinal dysbiosis is key in the onset and development of Crohn’s disease (CD). We evaluated the microbiota changes in CD patients before and after a six-month anti-TNF treatment, comparing these changes with the microbiota of healthy subjects. This prospective multicenter observational study involved 27 CD patients initiating anti-TNF treatment and 16 healthy individuals. Inflammatory activity was determined at baseline, 3 and 6 months, classifying patients into responders and non-responders. Fecal microbiota was analyzed by massive genomic sequencing thought 16S rRNA amplicon sequencing before and after six months of anti-TNF treatment. The CD cohort showed a decrease in genera of the class Clostridia, short-chain fatty acid producers, and an increase in the phylum Proteobacteria (p < 0.01) versus the healthy cohort. After anti-TNF treatment, the phylum Proteobacteria also increased in non-responders versus responders (13/27) (p < 0.005), with the class Clostridia increasing. In addition, alpha diversity increased in responders versus non-responders (p < 0.01), tending towards eubiosis. An association was found (p < 0.001) in the F.prausnitzii/E.coli ratio between responders and non-responders. The F/E ratio was the most accurate biomarker of anti-TNF response (area under the curve 0.87). Thus, anti-TNF treatment allows partial restoration of intestinal microbiota in responders and the F.prausnitzii/E.coli ratio can provide a reliable indicator of response to anti-TNF in CD.