Fundamentals of Determination of the Biological Tissue Refractive Index by Ellipsoidal Reflector Method
Natalia Bezugla,
Oleksandra Romodan,
Pawel Komada,
Nataliia Stelmakh,
Mykhailo Bezuglyi
Affiliations
Natalia Bezugla
Department of Computer-Integrated Technologies of Device Production, Faculty of Instrumentation Engineering, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Beresteiskyi Ave., 37, 03056 Kyiv, Ukraine
Oleksandra Romodan
Department of Computer-Integrated Technologies of Device Production, Faculty of Instrumentation Engineering, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Beresteiskyi Ave., 37, 03056 Kyiv, Ukraine
Pawel Komada
Department of Electronics and Information Techniques, Faculty of Electrical Engineering and Computer Science, Lublin University of Technology, 38D Nadbystrzycka Street, 20-618 Lublin, Poland
Nataliia Stelmakh
Department of Computer-Integrated Technologies of Device Production, Faculty of Instrumentation Engineering, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Beresteiskyi Ave., 37, 03056 Kyiv, Ukraine
Mykhailo Bezuglyi
Department of Computer-Integrated Technologies of Device Production, Faculty of Instrumentation Engineering, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Beresteiskyi Ave., 37, 03056 Kyiv, Ukraine
This paper presents the theoretical fundamentals, prerequisites for creation, and peculiarities of modeling a new method for determining the refractive index of biological tissues. The method uses a mirror ellipsoid of revolution as an optical element to ensure total internal reflection phenomena. This paper thoroughly analyzes the differences in the refractive index of healthy and pathological tissues on a biometric diagnostic basis. The analysis is used to model the measurement setup’s parameters. This paper also considers various methods of determining the refractive index of biological tissues based on different principles of physical optics, such as interferometry, refractometry, ellipsometry, and goniophotometry. It systematizes typical optical elements of total internal reflection that can be used in goniophotometry. It justifies the selection of the element base for the goniometric installation based on the ellipsoidal reflector method. A simulation of the installation operation was carried out for various parameters of the ellipsoidal reflector, ensuring the measurement of the biological tissue refractive index from 1.33 to 1.7. This paper also proposes a constructive solution for manufacturing an ellipsoidal reflector of the required configuration.
