Effect of Phage Spray on Hatchability and Chick Quality of Eggs Contaminated with <i>Salmonella</i> Typhimurium
Leping Wang,
Qinting Dong,
Kunping Tang,
Kaiou Han,
Huili Bai,
Yangyan Yin,
Changting Li,
Chunxia Ma,
Ling Teng,
Jun Li,
Yu Gong,
Yuying Liao,
Hao Peng,
Xiaoye Wang
Affiliations
Leping Wang
Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China
Qinting Dong
Guangxi Vocational University of Agriculture, Nanning 530009, China
Kunping Tang
Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China
Kaiou Han
Animal Disease Prevention and Control Center, Guilin 541000, China
Huili Bai
Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China
Yangyan Yin
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China
Changting Li
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China
Chunxia Ma
Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China
Ling Teng
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China
Jun Li
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China
Yu Gong
Animal Science and Technology Station of Guizhou, Guiyang 550018, China
Yuying Liao
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China
Hao Peng
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China
Xiaoye Wang
Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China
Salmonella Typhimurium (S. Typhimurium) contamination poses a significant challenge to breeder egg hatchability and chick health, necessitating the exploration of alternative disinfection methods. This study investigates the potential of phage vB_SPuM_SP02 (SP02) as a novel disinfectant for breeder eggs contaminated with S. Typhimurium SM022. Phage SP02 was isolated from poultry farm effluent and characterized for morphology, biological properties, and genome properties. Experimental groups of specific pathogen-free (SPF) eggs were treated with Salmonella and phage SP02, and efficacy was assessed through hatching rates, chick survival, weight, Salmonella load, immune organ indices, and intestinal flora. Phage treatment effectively eradicated Salmonella contamination on eggshells within 12 h, resulting in increased hatching and survival rates compared to controls. Furthermore, phage treatment mitigated weight loss and tissue Salmonella load in chicks without causing immune organ damage while reducing Salmonella spp. abundance in the intestinal tract. This study demonstrates the potential of phage SP02 as an eco-friendly and efficient disinfectant for S. Typhimurium-contaminated breeder eggs, offering promising prospects for practical application in poultry production.
