Effect of Flavonoid-Coated Gold Nanoparticles on Bacterial Colonization in Mice Organs
Sundus Riaz,
Nosheen Fatima Rana,
Irshad Hussain,
Tahreem Tanweer,
Afrah Nawaz,
Farid Menaa,
Hussnain A. Janjua,
Tahseen Alam,
Amna Batool,
Ayesha Naeem,
Maryam Hameed,
Syed Mohsin Ali
Affiliations
Sundus Riaz
Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, Islamabad 44000, Pakistan
Nosheen Fatima Rana
Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, Islamabad 44000, Pakistan
Irshad Hussain
School of Science and Engineering, Lahore University of Management Sciences, Lahore 54000, Pakistan
Tahreem Tanweer
Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, Islamabad 44000, Pakistan
Afrah Nawaz
Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, Islamabad 44000, Pakistan
Farid Menaa
Department of Internal Medicine and Nanomedicine, California Innovations Corporation, San Diego, CA 92093, USA
Hussnain A. Janjua
Atta Ur Rahman School of Applied Biosciences, National University of Sciences & Technology, Islamabad 44000, Pakistan
Tahseen Alam
Atta Ur Rahman School of Applied Biosciences, National University of Sciences & Technology, Islamabad 44000, Pakistan
Amna Batool
Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, Islamabad 44000, Pakistan
Ayesha Naeem
Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, Islamabad 44000, Pakistan
Maryam Hameed
Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, Islamabad 44000, Pakistan
Syed Mohsin Ali
Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Sciences & Technology, Islamabad 44000, Pakistan
Multidrug resistance (MDR) has been a potentiator for the exploration of antibiotics. Nano drug delivery systems have opened new avenues to overcome this challenge. Although antibacterial nanocarriers are extensively realized, their effect on the bacteria residing inside the tissues and their toxicity is rarely explored. This study investigated the effects of flavonoid coated gold nanoparticles (FAuNPs) on the colonization of Enterococcus faecalis in the mouse liver and kidneys. Flavonoids were extracted from the leaves of Berberis lycium Royle and used to stabilize gold following a green synthesis approach. FAuNPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared spectroscopy (FTIR), scanning transmission electron microscopy (STEM), X-ray powder diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDS). FAuNPs showed significantly higher reduction in bacterial counts in in-vitro and in-vivo in mice organs as compared to the free flavonoids owing to their biocompatibility and effectiveness.
