Rapid Screening of 60 Types of Natural Toxins in Whole Blood by UPLC-Q/Orbitrap HRMS

GUO Zhu-tao; DENG Qian-ya; HE Si-yang; ZHENG Shui-qing; WANG Rong; SUN Wen-juan; LIANG Chen; NI Chun-fang

doi:10.12116/j.issn.1004-5619.2024.340202

Fayixue Zazhi (Dec 2024)

Rapid Screening of 60 Types of Natural Toxins in Whole Blood by UPLC-Q/Orbitrap HRMS

GUO Zhu-tao,
DENG Qian-ya,
HE Si-yang,
ZHENG Shui-qing,
WANG Rong,
SUN Wen-juan,
LIANG Chen,
NI Chun-fang

Affiliations

GUO Zhu-tao: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China
DENG Qian-ya: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China
HE Si-yang: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China
ZHENG Shui-qing: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China
WANG Rong: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China
SUN Wen-juan: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China
LIANG Chen: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China
NI Chun-fang: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China

DOI: https://doi.org/10.12116/j.issn.1004-5619.2024.340202
Journal volume & issue: Vol. 40, no. 6
pp. 564 – 568

Abstract

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ObjectiveTo establish a rapid screening method for 60 types of natural toxins in whole blood by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry （UPLC-Q/Orbitrap HRMS).MethodsThe chromatographic and mass spectrometric information of 60 standard samples of natural toxins were analyzed and recorded, and a screening database was built. Whole blood was pretreated by protein precipitation method combined with ultrasonic-assisted dispersion, and then a Hypersil GOLDTM C18 column was used with 5 mmol/L ammonium formate aqueous solution （containing 0.1% formic acid） and acetonitrile as mobile phase for gradient elution. In the positive ion mode, the data were collected in full scan/data-dependent secondary scan （Full MS/dd-MS2） mode. Based on the established screening library, the rapid screening of 60 types of natural toxins in whole blood was realized by TraceFinder software.ResultsA UPLC-Q/Orbitrap HRMS method was developed for the screening of 60 types of natural toxins in whole blood. Except for the limit of detection （LOD） of oxymatrine （20 ng/mL） and strophanthidin （40 ng/mL）, the LOD for the other 58 natural toxins was in the range of 0.05-5 ng/mL.ConclusionThis method has a simple and efficient pretreatment process and can achieve rapid screening of 60 types of natural toxins in whole blood.

Published in Fayixue Zazhi

ISSN: 1004-5619 (Print)
Publisher: Editorial Office of Journal of Forensic Medicine
Country of publisher: China
LCC subjects: Medicine
Website: http://www.fyxzz.cn/EN/1004-5619/home.shtml

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