Фармакокинетика и Фармакодинамика (Apr 2020)

HPLC-MS method development and validation for simultaneous quantitation of GZK-111 and CPG compounds in rat plasma

  • A. L. Podolko,
  • P. O. Bochkov,
  • G. B. Kolyvanov,
  • A. A. Litvin,
  • O. G. Gribakina,
  • R. V. Shevchenko,
  • V. P. Zherdev

DOI
https://doi.org/10.37489/2587-7836-2019-4-28-36
Journal volume & issue
Vol. 0, no. 4
pp. 28 – 36

Abstract

Read online

The technique of quantitative determination of GZK-111 and CPG compounds in rat blood plasma has been developed. The analysis was carried out using the combined method of high-performance liquid chromatography with mass spectrometric detection. The method was linear in the concentration range of 25-1000 ng/ml for both compounds. Percentage of GZK-111 extraction from rat blood plasma was 57.8 %. The lower limit of detection for GZK- 111 compound was 25 ng/ml. Percentage of extraction of CPG compound was 42.5 %. The lower limit of detection of CPG compound was also 25 ng/ml.

Keywords