Fluorescent Characteristics and Metal Binding Properties of Different Molecular Weight Fractions in Stratified Extracellular Polymeric Substances of Activated Sludge

Abstract

The combination of heavy metals and extracellular polymeric substances (EPSs) affects the distribution of heavy metals in microbial aggregates, soil, and aquatic systems. This paper aimed to explore the binding mechanisms of EPSs of different molecular weights in activated sludge with heavy metals. We extracted the stratification components of activated sludge EPSs and divided the components into five fractions of different molecular weight ranges. In the three-dimensional fluorescence analysis of each fraction, the EPSs of activated sludge had two peaks, peak A (representing low-excitation tryptophan) and peak B (representing high-excitation tryptophan), and static quenching was the main reason for the fluorescence quenching between the compounds attributable to peak A in activated sludge EPSs and Pb2+ and Cu2+. Further exploration suggested that the EPSs of activated sludge interacted with Cd2+, Pb2+, Cu2+, and Zn2+ to form new substances. The quenching effect of the EPSs with the highest molecular weight (100 kDa–0.7 μm) was more significant, and the binding ability was more stable. This study implies that the application of EPSs from activated sludge is promising. While effectively binding heavy metals, it can also reduce the volume of the excess activated sludge.

Keywords

• extracellular polymeric substances (EPSs)
• different molecular weight
• heavy metal
• interaction mechanism
• fluorescence quenching