Inclusion of vitexin in β-cyclodextrin: preparation, characterization and expectorant/antitussive activities
Eliatania Clementino Costa,
Pedro Modesto Nascimento Menezes,
Ricardo Lúcio de Almeida,
Fabrício Souza Silva,
Luciano Augusto de Araújo Ribeiro,
James Amalda da Silva,
Ana Paula de Oliveira,
Edigênia Cavalcante da Cruz Araújo,
Larissa Araújo Rolim,
Xirley Pereira Nunes
Affiliations
Eliatania Clementino Costa
Rede Nordeste de Biostecnologia (RENORBIO), Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil; Corresponding author.
Pedro Modesto Nascimento Menezes
Rede Nordeste de Biostecnologia (RENORBIO), Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil
Ricardo Lúcio de Almeida
Pós-graduação em Biociências (PGB), Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, PE, Brazil
Fabrício Souza Silva
Pós-graduação em Biociências (PGB), Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, PE, Brazil
Luciano Augusto de Araújo Ribeiro
Pós-graduação em Biociências (PGB), Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, PE, Brazil
James Amalda da Silva
Universidade Federal de Sergipe (UFS), Av. Gov. Marcelo Déda, São José, Lagarto, SE, Brazil
Ana Paula de Oliveira
Rede Nordeste de Biostecnologia (RENORBIO), Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil; Pós-graduação em Biociências (PGB), Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, PE, Brazil
Edigênia Cavalcante da Cruz Araújo
Pós-graduação em Biociências (PGB), Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, PE, Brazil
Larissa Araújo Rolim
Rede Nordeste de Biostecnologia (RENORBIO), Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil; Pós-graduação em Biociências (PGB), Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, PE, Brazil
Xirley Pereira Nunes
Rede Nordeste de Biostecnologia (RENORBIO), Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil; Pós-graduação em Biociências (PGB), Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, PE, Brazil
The study aimed to include the isolated vitexin of Jatropha mutabilis in the β-cyclodextrin cavity to improve the solubility of this flavone. Its characterization was performed by techniques such as 1H NMR/ROESY (Nuclear Magnetic Resonance Spectroscopy), FT-IR (Infrared Spectroscopy with Fourier Transform), SEM (Morphological analysis of IC by Scanning Electron Microscopy) and dissolution study in vitro. In addition, the following activities were evaluated in the animal models: expectorant, phenol red dosage in bronchoalveolar lavage and antitussive, cough induced by citric acid. In the characterization of the complex, interaction between hydrogens of ring B of vitexin and (H3) of β-CD was observed, in addition to changes in morphology. In the dissolution test, an increase in the rate of dissolution of vitexin was observed in the first 30 min for the CI vitexin/β-CD when compared with vitexin. Regarding the pharmacological activity, it was observed that the inclusion complex (IC) vitexin/β-CD in the equivalent doses of 0.2, 1 and 5 mg/kg of flavone presented higher expectorant activity when compared to vitexin (p < 0.05), suggesting increased bioavailability. As for the antitussive activity, both vitexin and the complex had similar effects and were dose independent. In the toxicity test using Artemia salina, vitexin and IC vitexin/β-CD were considered non-toxic. At last, the study efficacy of vitexin/β-CD IC as an expectorant and of vitexin as antitussive. All of these data are being described for the first time.
