OsMADS58 Stabilizes Gene Regulatory Circuits during Rice Stamen Development
Liping Shen,
Feng Tian,
Zhukuan Cheng,
Qiang Zhao,
Qi Feng,
Yan Zhao,
Bin Han,
Yuhan Fang,
Yanan Lin,
Rui Chen,
Donghui Wang,
Wenfeng Sun,
Jiaqi Sun,
Hongyun Zeng,
Nan Yao,
Ge Gao,
Jingchu Luo,
Zhihong Xu,
Shunong Bai
Affiliations
Liping Shen
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Feng Tian
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Zhukuan Cheng
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
Qiang Zhao
National Center for Gene Research, State Key Laboratory of Plant Molecular Genetics, CAS Center of Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200233, China
Qi Feng
National Center for Gene Research, State Key Laboratory of Plant Molecular Genetics, CAS Center of Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200233, China
Yan Zhao
National Center for Gene Research, State Key Laboratory of Plant Molecular Genetics, CAS Center of Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200233, China
Bin Han
National Center for Gene Research, State Key Laboratory of Plant Molecular Genetics, CAS Center of Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200233, China
Yuhan Fang
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Yanan Lin
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Rui Chen
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Donghui Wang
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Wenfeng Sun
Novogene, Beijing 100015, China
Jiaqi Sun
School of Life Science, Sun Yat-sen University, Guangzhou 510260, China
Hongyun Zeng
School of Life Science, Sun Yat-sen University, Guangzhou 510260, China
Nan Yao
School of Life Science, Sun Yat-sen University, Guangzhou 510260, China
Ge Gao
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Jingchu Luo
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Zhihong Xu
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Shunong Bai
State Key Laboratory of Protein & Plant Gene Research, Peking University, Beijing 100871, China
Rice (Oryza sativa) OsMADS58 is a C-class MADS box protein, and characterization of a transposon insertion mutant osmads58 suggested that OsMADS58 plays a role in stamen development. However, as no null mutation has been obtained, its role has remained unclear. Here, we report that the CRISPR knockout mutant osmads58 exhibits complex altered phenotypes, including anomalous diploid germ cells, aberrant meiosis, and delayed tapetum degeneration. This CRISPR mutant line exhibited stronger changes in expression of OsMADS58 target genes compared with the osmads58 dSpm (transposon insertion) line, along with changes in multiple pathways related to early stamen development. Notably, transcriptional regulatory circuits in young panicles covering the stamen at stages 4–6 were substantially altered in the CRISPR line compared to the dSpm line. These findings strongly suggest that the pleiotropic effects of OsMADS58 on stamen development derive from a potential role in stabilizing gene regulatory circuits during early stamen development. Thus, this work opens new avenues for viewing and deciphering the regulatory mechanisms of early stamen development from a network perspective.
