In Vivo Study of Nasal Bone Reconstruction with Collagen, Elastin and Chitosan Membranes in Abstainer and Alcoholic Rats
Fabricio Egidio Pandini,
Fabíola Mayumi Miyauchi Kubo,
Ana Maria de Guzzi Plepis,
Virginia da Conceição Amaro Martins,
Marcelo Rodrigues da Cunha,
Vinicius Rodrigues Silva,
Vinicius Barroso Hirota,
Everton Lopes,
Marcos Antonio Menezes,
André Antonio Pelegrine,
Tiago Negrão de Andrade,
Amilton Iatecola,
Bruna da Cruz Britto,
Victor Augusto Ramos Fernandes,
Luis Felipe Orsi Gameiro,
Ronny Rodrigues Correia,
Marcelo Lucchesi Teixeira,
Getúlio Duarte Júnior,
Carlos Henrique Bertoni Reis,
Eliana de Souza Bastos Mazuqueli Pereira,
Daniela Vieira Buchaim,
Karina Torres Pomini,
Daniel de Bortoli Teixeira,
Rogerio Leone Buchaim,
Edmir Américo Lourenço
Affiliations
Fabricio Egidio Pandini
Department of Surgery (Otorhinolaryngology), Jundiaí Medical School, Jundiaí 13202-550, Brazil
Fabíola Mayumi Miyauchi Kubo
Department of Implant Dentistry, Faculdade São Leopoldo Mandic, Campinas 13045-755, Brazil
Ana Maria de Guzzi Plepis
Interunit Postgraduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo (USP), São Carlos 13566-590, Brazil
Virginia da Conceição Amaro Martins
São Carlos Institute of Chemistry, University of São Paulo (USP), São Carlos 13566-590, Brazil
Marcelo Rodrigues da Cunha
Interunit Postgraduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo (USP), São Carlos 13566-590, Brazil
Vinicius Rodrigues Silva
Department of Morphology and Pathology, Jundiaí Medical School, Jundiaí 13202-550, Brazil
Vinicius Barroso Hirota
Coordination of the Physical Education Course, University Center of the Americas (FAM), São Paulo 01304-001, Brazil
Everton Lopes
Padre Anchieta University Center, Jundiaí 13210-795, Brazil
Marcos Antonio Menezes
Department of Implant Dentistry, Faculdade São Leopoldo Mandic, Campinas 13045-755, Brazil
André Antonio Pelegrine
Department of Implant Dentistry, Faculdade São Leopoldo Mandic, Campinas 13045-755, Brazil
Tiago Negrão de Andrade
Department of Morphology and Pathology, Jundiaí Medical School, Jundiaí 13202-550, Brazil
Amilton Iatecola
Department of Morphology and Pathology, Jundiaí Medical School, Jundiaí 13202-550, Brazil
Bruna da Cruz Britto
Department of Morphology and Pathology, Jundiaí Medical School, Jundiaí 13202-550, Brazil
Victor Augusto Ramos Fernandes
Department of Morphology and Pathology, Jundiaí Medical School, Jundiaí 13202-550, Brazil
Luis Felipe Orsi Gameiro
Unimetrocamp University Center, Campinas 13035-500, Brazil
Ronny Rodrigues Correia
Botucatu Medical School (FMB), São Paulo State University (UNESP—Univ Estadual Paulista), Botucatu 18618-687, Brazil
Marcelo Lucchesi Teixeira
Prosthodontics Department, Faculdade São Leopoldo Mandic, Campinas 13045-755, Brazil
Getúlio Duarte Júnior
Unimar Beneficent Hospital (HBU), University of Marilia (UNIMAR), Marilia 17525-160, Brazil
Carlos Henrique Bertoni Reis
Unimar Beneficent Hospital (HBU), University of Marilia (UNIMAR), Marilia 17525-160, Brazil
Eliana de Souza Bastos Mazuqueli Pereira
Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marília 17525-902, Brazil
Daniela Vieira Buchaim
Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marília 17525-902, Brazil
Karina Torres Pomini
Department of Biological Sciences, Bauru School of Dentistry (FOB/USP), University of São Paulo, Bauru 17012-901, Brazil
Daniel de Bortoli Teixeira
Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marília 17525-902, Brazil
Rogerio Leone Buchaim
Department of Biological Sciences, Bauru School of Dentistry (FOB/USP), University of São Paulo, Bauru 17012-901, Brazil
Edmir Américo Lourenço
Department of Surgery (Otorhinolaryngology), Jundiaí Medical School, Jundiaí 13202-550, Brazil
The aim of the present study was to evaluate the use of collagen, elastin, or chitosan biomaterial for bone reconstruction in rats submitted or not to experimental alcoholism. Wistar male rats were divided into eight groups, submitted to chronic alcohol ingestion (G5 to G8) or not (G1 to G4). Nasal bone defects were filled with clot in animals of G1 and G5 and with collagen, elastin, and chitosan grafts in G2/G6, G3/G7, and G4/G8, respectively. Six weeks after, all specimens underwent radiographic, tomographic, and microscopic evaluations. Bone mineral density was lower in the defect area in alcoholic animals compared to the abstainer animals. Bone neoformation was greater in the abstainer groups receiving the elastin membrane and in abstainer and alcoholic rats receiving the chitosan membrane (15.78 ± 1.19, 27.81 ± 0.91, 47.29 ± 0.97, 42.69 ± 1.52, 13.81 ± 1.60, 18.59 ± 1.37, 16.54 ± 0.89, and 37.06 ± 1.17 in G1 to G8, respectively). In conclusion, osteogenesis and bone density were more expressive after the application of the elastin matrix in abstainer animals and of the chitosan matrix in both abstainer and alcoholic animals. Chronic alcohol ingestion resulted in lower bone formation and greater formation of fibrous connective tissue.