Protocol for fluorescent live-cell staining of tardigrades

Clayton J. Harry; Jonathan D. Hibshman; Amor Damatac, II; Phillip L. Davidson; Martin Andres Estermann; Marycruz Flores-Flores; Caroline M. Holmes; Jorge Lázaro; Elizabeth-Ann Legere; Jake Leyhr; Siddharthan Balachandar Thendral; Bridget A. Vincent; Bob Goldstein

STAR Protocols (Sep 2024)

Protocol for fluorescent live-cell staining of tardigrades

Clayton J. Harry,
Jonathan D. Hibshman,
Amor Damatac, II,
Phillip L. Davidson,
Martin Andres Estermann,
Marycruz Flores-Flores,
Caroline M. Holmes,
Jorge Lázaro,
Elizabeth-Ann Legere,
Jake Leyhr,
Siddharthan Balachandar Thendral,
Bridget A. Vincent,
Bob Goldstein

Affiliations

Clayton J. Harry: Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Jonathan D. Hibshman: Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA
Amor Damatac, II: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Max Planck Institute for Evolutionary Biology, 24306 Ploen, Germany
Phillip L. Davidson: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Biology, Indiana University, Bloomington, IN 47405, USA
Martin Andres Estermann: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Reproductive Developmental Biology Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
Marycruz Flores-Flores: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; University of Colorado, School of Medicine, Anschutz Medical Campus, Department of Pediatrics, Section of Developmental Biology, 12801 E 17th Avenue, Aurora, CO 80045, USA
Caroline M. Holmes: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Organismal and Evolutionary Biology, Harvard University, Boston, MA, USA
Jorge Lázaro: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; European Molecular Biology Laboratory (EMBL) Barcelona, Dr. Aiguader 88, 08003 Barcelona, Spain
Elizabeth-Ann Legere: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Human Genetics, McGill University, Montreal, QC, Canada
Jake Leyhr: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Organismal Biology, Uppsala University, Uppsala, Sweden; Department of Biology, Duke University, Durham, NC 27708, USA
Siddharthan Balachandar Thendral: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Biology, Duke University, Durham, NC 27708, USA
Bridget A. Vincent: Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Ecology, Evolution, and Marine Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
Bob Goldstein: Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Embryology Course, Marine Biological Laboratory, Woods Hole, MA 02543, USA; Corresponding author

Journal volume & issue: Vol. 5, no. 3
p. 103232

Abstract

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Summary: Tardigrades are microscopic organisms with exceptional resilience to environmental extremes. Most protocols to visualize the internal anatomy of tardigrades rely on fixation, hampering our understanding of dynamic changes to organelles and other subcellular components. Here, we provide protocols for staining live tardigrade adults and other postembryonic stages, facilitating real-time visualization of structures including lipid droplets, mitochondria, lysosomes, and DNA. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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