Journal of Lipid Research (Dec 2017)
Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950–Metabolites in Frozen Human Plasma[S]
- John A. Bowden,
- Alan Heckert,
- Candice Z. Ulmer,
- Christina M. Jones,
- Jeremy P. Koelmel,
- Laila Abdullah,
- Linda Ahonen,
- Yazen Alnouti,
- Aaron M. Armando,
- John M. Asara,
- Takeshi Bamba,
- John R. Barr,
- Jonas Bergquist,
- Christoph H. Borchers,
- Joost Brandsma,
- Susanne B. Breitkopf,
- Tomas Cajka,
- Amaury Cazenave-Gassiot,
- Antonio Checa,
- Michelle A. Cinel,
- Romain A. Colas,
- Serge Cremers,
- Edward A. Dennis,
- James E. Evans,
- Alexander Fauland,
- Oliver Fiehn,
- Michael S. Gardner,
- Timothy J. Garrett,
- Katherine H. Gotlinger,
- Jun Han,
- Yingying Huang,
- Aveline Huipeng Neo,
- Tuulia Hyötyläinen,
- Yoshihiro Izumi,
- Hongfeng Jiang,
- Houli Jiang,
- Jiang Jiang,
- Maureen Kachman,
- Reiko Kiyonami,
- Kristaps Klavins,
- Christian Klose,
- Harald C. Köfeler,
- Johan Kolmert,
- Therese Koal,
- Grielof Koster,
- Zsuzsanna Kuklenyik,
- Irwin J. Kurland,
- Michael Leadley,
- Karen Lin,
- Krishna Rao Maddipati,
- Danielle McDougall,
- Peter J. Meikle,
- Natalie A. Mellett,
- Cian Monnin,
- M. Arthur Moseley,
- Renu Nandakumar,
- Matej Oresic,
- Rainey Patterson,
- David Peake,
- Jason S. Pierce,
- Martin Post,
- Anthony D. Postle,
- Rebecca Pugh,
- Yunping Qiu,
- Oswald Quehenberger,
- Parsram Ramrup,
- Jon Rees,
- Barbara Rembiesa,
- Denis Reynaud,
- Mary R. Roth,
- Susanne Sales,
- Kai Schuhmann,
- Michal Laniado Schwartzman,
- Charles N. Serhan,
- Andrej Shevchenko,
- Stephen E. Somerville,
- Lisa St. John-Williams,
- Michal A. Surma,
- Hiroaki Takeda,
- Rhishikesh Thakare,
- J. Will Thompson,
- Federico Torta,
- Alexander Triebl,
- Martin Trötzmüller,
- S. J. Kumari Ubhayasekera,
- Dajana Vuckovic,
- Jacquelyn M. Weir,
- Ruth Welti,
- Markus R. Wenk,
- Craig E. Wheelock,
- Libin Yao,
- Min Yuan,
- Xueqing Heather Zhao,
- Senlin Zhou
Affiliations
- John A. Bowden
- Marine Biochemical Sciences Group, Chemical Sciences Division, Hollings Marine Laboratory, National Institute of Standards and Technology, Charleston, SC; To whom correspondence should be addressed. e-mail:
- Alan Heckert
- Statistical Engineering Division, National Institute of Standards and Technology, Gaithersburg, MD
- Candice Z. Ulmer
- Marine Biochemical Sciences Group, Chemical Sciences Division, Hollings Marine Laboratory, National Institute of Standards and Technology, Charleston, SC
- Christina M. Jones
- Marine Biochemical Sciences Group, Chemical Sciences Division, Hollings Marine Laboratory, National Institute of Standards and Technology, Charleston, SC
- Jeremy P. Koelmel
- Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL
- Laila Abdullah
- The Roskamp Institute, Sarasota, FL
- Linda Ahonen
- Steno Diabetes Center Copenhagen, Gentofte, Denmark
- Yazen Alnouti
- Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE
- Aaron M. Armando
- Departments of Chemistry and Biochemistry and Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA
- John M. Asara
- Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA; Department of Medicine, Harvard Medical School, Boston, MA
- Takeshi Bamba
- Division of Metabolomics, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka, Japan
- John R. Barr
- Division of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA
- Jonas Bergquist
- Department of Chemistry-BMC, Analytical Chemistry, Uppsala University, Uppsala, Sweden
- Christoph H. Borchers
- University of Victoria-Genome British Columbia Proteomics Centre, University of Victoria, Victoria, British Columbia, Canada; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada; Gerald Bronfman Department of Oncology McGill University, Montreal, Quebec, Canada; Proteomics Centre, Segal Cancer Centre, Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, Quebec, Canada
- Joost Brandsma
- Faculty of Medicine, Academic Unit of Clinical and Experimental Sciences, Southampton General Hospital, University of Southampton, Southampton, United Kingdom
- Susanne B. Breitkopf
- Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA
- Tomas Cajka
- National Institutes of Health West Coast Metabolomics Center, University of California Davis Genome Center, Davis, CA
- Amaury Cazenave-Gassiot
- Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore and Singapore Lipidomic Incubator (SLING), Life Sciences Institute, Singapore
- Antonio Checa
- Division of Physiological Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
- Michelle A. Cinel
- Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia
- Romain A. Colas
- Department of Anesthesiology, Perioperative and Pain Medicine, Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital and Harvard Medical School, Boston, MA
- Serge Cremers
- Biomarker Core Laboratory, Irving Institute for Clinical and Translational Research, Columbia University Medical Center, New York, NY
- Edward A. Dennis
- Departments of Chemistry and Biochemistry and Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA
- James E. Evans
- The Roskamp Institute, Sarasota, FL
- Alexander Fauland
- Division of Physiological Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
- Oliver Fiehn
- National Institutes of Health West Coast Metabolomics Center, University of California Davis Genome Center, Davis, CA; Biochemistry Department, King Abdulaziz University, Jeddah, Saudi Arabia
- Michael S. Gardner
- Division of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA
- Timothy J. Garrett
- Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL
- Katherine H. Gotlinger
- Department of Pharmacology, New York Medical College School of Medicine, Valhalla, NY
- Jun Han
- University of Victoria-Genome British Columbia Proteomics Centre, University of Victoria, Victoria, British Columbia, Canada
- Yingying Huang
- Thermo Fisher Scientific, San Jose, CA
- Aveline Huipeng Neo
- Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore and Singapore Lipidomic Incubator (SLING), Life Sciences Institute, Singapore
- Tuulia Hyötyläinen
- Department of Chemistry, Örebro University, Örebro, Sweden
- Yoshihiro Izumi
- Division of Metabolomics, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka, Japan
- Hongfeng Jiang
- Biomarker Core Laboratory, Irving Institute for Clinical and Translational Research, Columbia University Medical Center, New York, NY
- Houli Jiang
- Department of Pharmacology, New York Medical College School of Medicine, Valhalla, NY
- Jiang Jiang
- Departments of Chemistry and Biochemistry and Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA
- Maureen Kachman
- Metabolomics Core, BRCF, University of Michigan, Ann Arbor, MI
- Reiko Kiyonami
- Thermo Fisher Scientific, San Jose, CA
- Kristaps Klavins
- Biocrates Life Sciences AG, Innsbruck, Austria
- Christian Klose
- Lipotype GmbH, Dresden, Germany
- Harald C. Köfeler
- Core Facility for Mass Spectrometry, Medical University of Graz, Graz, Austria
- Johan Kolmert
- Division of Physiological Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
- Therese Koal
- Biocrates Life Sciences AG, Innsbruck, Austria
- Grielof Koster
- Faculty of Medicine, Academic Unit of Clinical and Experimental Sciences, Southampton General Hospital, University of Southampton, Southampton, United Kingdom
- Zsuzsanna Kuklenyik
- Division of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA
- Irwin J. Kurland
- Stable Isotope and Metabolomics Core Facility, Diabetes Research Center, Albert Einstein College of Medicine, Bronx, NY
- Michael Leadley
- Analytical Facility of Bioactive Molecules, The Hospital for Sick Children Research Institute, Toronto, ON, Canada
- Karen Lin
- University of Victoria-Genome British Columbia Proteomics Centre, University of Victoria, Victoria, British Columbia, Canada
- Krishna Rao Maddipati
- Lipidomics Core Facility and Department of Pathology, Wayne State University, Detroit, MI
- Danielle McDougall
- Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL
- Peter J. Meikle
- Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia
- Natalie A. Mellett
- Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia
- Cian Monnin
- Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, Canada
- M. Arthur Moseley
- Proteomics and Metabolomics Shared Resource, Levine Science Research Center, Duke University School of Medicine, Durham, NC
- Renu Nandakumar
- Biomarker Core Laboratory, Irving Institute for Clinical and Translational Research, Columbia University Medical Center, New York, NY
- Matej Oresic
- Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland
- Rainey Patterson
- Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL
- David Peake
- Thermo Fisher Scientific, San Jose, CA
- Jason S. Pierce
- Department of Biochemistry and Molecular Biology Medical University of South Carolina, Charleston, SC
- Martin Post
- Analytical Facility of Bioactive Molecules, The Hospital for Sick Children Research Institute, Toronto, ON, Canada
- Anthony D. Postle
- Faculty of Medicine, Academic Unit of Clinical and Experimental Sciences, Southampton General Hospital, University of Southampton, Southampton, United Kingdom
- Rebecca Pugh
- Chemical Sciences Division, Environmental Specimen Bank Group, Hollings Marine Laboratory, National Institute of Standards and Technology, Charleston, SC
- Yunping Qiu
- Stable Isotope and Metabolomics Core Facility, Diabetes Research Center, Albert Einstein College of Medicine, Bronx, NY
- Oswald Quehenberger
- Departments of Medicine and Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA
- Parsram Ramrup
- Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, Canada
- Jon Rees
- Division of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA
- Barbara Rembiesa
- Department of Biochemistry and Molecular Biology Medical University of South Carolina, Charleston, SC
- Denis Reynaud
- Analytical Facility of Bioactive Molecules, The Hospital for Sick Children Research Institute, Toronto, ON, Canada
- Mary R. Roth
- Division of Biology, Kansas Lipidomics Research Center, Kansas State University, Manhattan, KS
- Susanne Sales
- Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
- Kai Schuhmann
- Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
- Michal Laniado Schwartzman
- Department of Pharmacology, New York Medical College School of Medicine, Valhalla, NY
- Charles N. Serhan
- Department of Anesthesiology, Perioperative and Pain Medicine, Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital and Harvard Medical School, Boston, MA
- Andrej Shevchenko
- Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
- Stephen E. Somerville
- Hollings Marine Laboratory, Medical University of South Carolina, Charleston, SC
- Lisa St. John-Williams
- Proteomics and Metabolomics Shared Resource, Levine Science Research Center, Duke University School of Medicine, Durham, NC
- Michal A. Surma
- Lipotype GmbH, Dresden, Germany
- Hiroaki Takeda
- Division of Metabolomics, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka, Japan
- Rhishikesh Thakare
- Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE
- J. Will Thompson
- Proteomics and Metabolomics Shared Resource, Levine Science Research Center, Duke University School of Medicine, Durham, NC
- Federico Torta
- Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore and Singapore Lipidomic Incubator (SLING), Life Sciences Institute, Singapore
- Alexander Triebl
- Core Facility for Mass Spectrometry, Medical University of Graz, Graz, Austria
- Martin Trötzmüller
- Core Facility for Mass Spectrometry, Medical University of Graz, Graz, Austria
- S. J. Kumari Ubhayasekera
- Department of Chemistry-BMC, Analytical Chemistry, Uppsala University, Uppsala, Sweden
- Dajana Vuckovic
- Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, Canada
- Jacquelyn M. Weir
- Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia
- Ruth Welti
- Division of Biology, Kansas Lipidomics Research Center, Kansas State University, Manhattan, KS
- Markus R. Wenk
- Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore and Singapore Lipidomic Incubator (SLING), Life Sciences Institute, Singapore
- Craig E. Wheelock
- Division of Physiological Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
- Libin Yao
- Division of Biology, Kansas Lipidomics Research Center, Kansas State University, Manhattan, KS
- Min Yuan
- Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA
- Xueqing Heather Zhao
- Stable Isotope and Metabolomics Core Facility, Diabetes Research Center, Albert Einstein College of Medicine, Bronx, NY
- Senlin Zhou
- Lipidomics Core Facility and Department of Pathology, Wayne State University, Detroit, MI
- Journal volume & issue
-
Vol. 58,
no. 12
pp. 2275 – 2288
Abstract
As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950–Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra- and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.
