Using Multiplex Polymerase Chain Reaction (m-PCR) Methods to detect six Pathogenic Bacteria in Human Oral Cavity

Abstract

According to estimates, the human oral cavity can harbour 700–1000 different species of bacteria, which are associated with periodontal and dental problems such as dental caries. Therefore, bacterial infections in the oral cavity may lead to significant health consequences, therefore early and correct identification is essential for effective treatment. This study has tried to create a multiplex PCR (m-PCR) procedure to detect six pathogenic bacteria frequently found in oral cavity infections: Streptococcus oralis, Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Veillonella parvula. To confirm the sensitivity of the m-PCR assay, the assay was able to detect as little as 250 pg/μL of genomic DNA from each target bacterium, demonstrating high sensitivity. In conclusion, we have developed a rapid and reliable m-PCR assay for the simultaneous detection of six pathogenic bacteria frequently found in oral cavity infections. This assay has the potential to be a valuable tool for the early and accurate diagnosis of oral cavity bacterial infections, leading to more effective treatment and improved patient outcomes.

Keywords

• oral cavity bacterial,,
• ,،biofilm,,
• ,،periodontal diseases,,
• ,،multiplex pcr