Ex vivo electrochemical measurement of glutamate release during spinal cord injury

Abstract

Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from ex vivo rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors. Protocol name: Electrochemical Glutamate Sensing from Resected Spinal Cord Segment, Keywords: Biosensor, SCI, Excitotoxicity, Direct ink writing, Additive manufacturing, Rapid prototyping, Implantable