Screening of Lactobacillus Fermentum from Piglets and Its Potential Probiotic Properties

Abstract

【Objective】A strain of Lactobacillus fermentum was isolated from the feces of healthy piglets, and its probiotic properties were tested in order to provide references for its applications in livestock and poultry breeding.【Method】106 strains of lactic acid bacteria were isolated by using Rogosa SL medium, and one strain which was potential for feeding was preliminarily screened by gram staining, acid and bile salt tolerance tests. The probiotic ability of strain LF1, which was identified as L. fermentum LF1 by 16S rDNA sequencing, was evaluated, including its antagonistic activity (Escherichia coli K88, Staphylococcus aureus, Streptococcus suis, Salmonella, and Vibrio, etc.), tolerance to the stimulated acidic environment in stomach and bile acid, and adhesion to small intestinal epithelial cells.【Result】L. fermentum LF1 had strong tolerance to acid and bile salt, showing its strong probiotic potential. However, the LF1 strain showed low adhesion to pig intestinal epithelial cells. In terms of antagonism, the culture supernatant of L. fermentum LF1 inhibited the growth of many animal pathogens significantly.【Conclusion】L. fermentum LF1 had a good probiotics ability. After further experimental evaluation on animals, it can be used as a probiotic candidate strain for livestock and poultry breeding industry.

Keywords

• lactobacillus fermentum
• probiotic property
• acid tolerance
• bile salt tolerance
• pathogen antagonism