Phytomedicine Plus (May 2024)
Biochemical and Histoarchitectural evaluation of 4-Vinylcyclohexane induced ovarian cancer against Alpinia purpurata (Vieill). K. Schum
Abstract
Objective: Alpinia purpurata is being studied for its potential to treat various conditions, including diabetes, arthritis, and tuberculosis. This research explores the impact of Alpinia purpurata (Vieill). K. Schum on ovarian cancer induced by 4-vinyl cyclohexane in Wistar albino rats. Materials and methods: Five sets of 100–120 g Wistar albino rats were assembled. Group 1 was the control group. Group 2 received intraperitoneal injection of 4-vinyl cyclohexane (80 mg/kg) for one month. Group 3 Treated similarly to Group 2 subsequently for the next two months treated with oral administration of A. purpurata ethyl acetate leaf extract (200 mg/kg body weight) Group 4 Treated similarly to Group subsequently for the next two months treated with Standard Drug Cisplatin (5 mg/kg body weight) weekly twice intraperitoneally. Group 5 acquired daily oral A. purpurata leaf extract (200 mg/kg) for two months. The rats were euthanized after the experiment under light chloroform anesthesia. Ovary and liver samples were obtained for lipid peroxidation, anti-oxidants, membrane-bound enzymes, tumor indicators, and histological investigation. Results: Over a 60-day period, rats were given an ethyl acetate extract of A. purpurata at a dose of 200 mg/kg, which lead to in a substantial (p < 0.05) increase in body protein content, as well as enzyme levels. Furthermore, the use of the ethyl acetate extract significantly (p < 0.05) recovered the altered lipid peroxidation activities in the ovarian tissues of both control and experimental rats to near-normal levels. These data imply that the extract has the capacity to quench free radicals, indicating possible anticancer effects. Conclusion: The results suggested that, the ethyl acetate extract of A. purpurata exhibited significant antitumor activity against 4-vinyl cyclohexane induced ovarian cancer bearing rats.
