Antimutagenic effects of 2,6-Dimethylpyridine-N-oxide using fluctuation ames test

Abstract

The mutagenic potential of chemical agents is a significant concern in the field of genetic toxicology. The 2,6-Dimethylpyridine-N-oxide is believed to have antimutagenic properties, which could be beneficial for applications in pharmaceutical and environmental sciences. This study aims to evaluate the antigenotoxic potential of 2,6-Dimethylpyridine-N-oxide using the fluctuation Ames test, a reliable method for detecting mutagenic effect. Specifically, the Ames assay was conducted with preincubation in a suspension of reversion Salmonella bacteria. The experimental approach utilized Salmonella typhimurium tester strains - TA98, which are characterized by frameshift mutations in the hisD3052 gene, and another strain TA100 featuring base-pair substitution mutations in the hisG46 gene to assess the genotoxic potential of the test compounds. The aim was to evaluate the antigenotoxic potential, these strains were concurrently exposed to positive mutagens: 4-Nitroquinoline-N-oxide (4-NQO) for the strain TA100 and 2-Nitrofluorene (2-NF) for TA98, without the presence of rat liver S9 microsomal fractions. Additionally, 2-Aminoanthracene (2-AA) was used for both strains with S9 activation alongside 2,6-Dimethylpyridine-N-oxide with the same dose level as positive controls by volume. The results indicate that 2,6-Dimethylpyridine-N-oxide exhibits significant antimutagenic effect, as shown by a notable reduction in mutation rates detected by the fluctuation Ames test. These findings support further investigation into the role of 2,6-Dimethylpyridine-N-oxide in preventing mutagenesis and its potential utility in drug development and environmental protection. This research provides essential insights into anti-mutagenesis and highlights the potential of 2,6-Dimethylpyridine-N-oxide in mitigating genotoxic risks.

Keywords

• 2.6-Dimethylpyridine-N-oxide
• Antimutagenic effect
• Fluctuation Ames Test