Nature Communications (Dec 2021)
Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution
- Ana Martinez-Val,
- Dorte B. Bekker-Jensen,
- Sophia Steigerwald,
- Claire Koenig,
- Ole Østergaard,
- Adi Mehta,
- Trung Tran,
- Krzysztof Sikorski,
- Estefanía Torres-Vega,
- Ewa Kwasniewicz,
- Sólveig Hlín Brynjólfsdóttir,
- Lisa B. Frankel,
- Rasmus Kjøbsted,
- Nicolai Krogh,
- Alicia Lundby,
- Simon Bekker-Jensen,
- Fridtjof Lund-Johansen,
- Jesper V. Olsen
Affiliations
- Ana Martinez-Val
- Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen
- Dorte B. Bekker-Jensen
- Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen
- Sophia Steigerwald
- Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen
- Claire Koenig
- Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen
- Ole Østergaard
- Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen
- Adi Mehta
- Department of Immunology, Oslo University Hospital, Rikshospitalet
- Trung Tran
- Department of Immunology, Oslo University Hospital, Rikshospitalet
- Krzysztof Sikorski
- Department of Immunology, Oslo University Hospital, Rikshospitalet
- Estefanía Torres-Vega
- Cardiac Proteomics, Faculty of Health and Medical Sciences, University of Copenhagen
- Ewa Kwasniewicz
- Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen
- Sólveig Hlín Brynjólfsdóttir
- Danish Cancer Society
- Lisa B. Frankel
- Danish Cancer Society
- Rasmus Kjøbsted
- The August Krogh Section for Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen
- Nicolai Krogh
- Department of Cellular and Molecular Medicine, University of Copenhagen
- Alicia Lundby
- Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen
- Simon Bekker-Jensen
- Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen
- Fridtjof Lund-Johansen
- Department of Immunology, Oslo University Hospital, Rikshospitalet
- Jesper V. Olsen
- Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen
- DOI
- https://doi.org/10.1038/s41467-021-27398-y
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 17
Abstract
Protein activity regulated by phosphorylation can result in subcellular relocation. Here, the authors present a high throughput spatial phosphoproteomics approach to profile six subcellular compartments, providing insights into EGFR and stress signalling dynamics.
