Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium

Si-yang LIU; Ren-yong JIA; Qing-qing LI; Dai-shen FENG; Hao-yue SHEN; Cui YANG; Ming-shu WANG; De-kang ZHU; Shun CHEN; Ma-feng LIU; Xin-xin ZHAO; Zhong-qiong YIN; Bo JING; An-chun CHENG

Journal of Integrative Agriculture (Apr 2018)

Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium

Si-yang LIU,
Ren-yong JIA,
Qing-qing LI,
Dai-shen FENG,
Hao-yue SHEN,
Cui YANG,
Ming-shu WANG,
De-kang ZHU,
Shun CHEN,
Ma-feng LIU,
Xin-xin ZHAO,
Zhong-qiong YIN,
Bo JING,
An-chun CHENG

Affiliations

Si-yang LIU: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Ren-yong JIA: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China; Correspondence JIA Ren-yong, Tel/Fax: +86-28-86291176
Qing-qing LI: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Dai-shen FENG: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Hao-yue SHEN: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Cui YANG: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Ming-shu WANG: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
De-kang ZHU: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Shun CHEN: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Ma-feng LIU: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Xin-xin ZHAO: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Zhong-qiong YIN: Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
Bo JING: Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China
An-chun CHENG: Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China

Journal volume & issue: Vol. 17, no. 4
pp. 928 – 939

Abstract

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Duck hepatitis B virus (DHBV) shares many basic characteristics with hepatitis B virus (HBV) and is an attractive model for vaccine development. In this study, DHBV DNA vaccines were designed to express envelope and capsid fusion proteins to enhance the breadth of immune response in ducks. Attenuated Salmonella typhimurium (SL7207) was used as a carrier and adjuvant to boost the magnitude of immune response. Based on this strategy, novel DNA vaccines (SL7207-pVAX1-LC and SL7207-pVAX1-SC) were generated. Growth kinetics, genetic stabilities and relative transcription levels of the L, S and C genes introduced by these vaccine strains were measured before inoculation to guarantee safety and efficacy. The relative transcript levels of the CD4 and CD8 T genes and the antibody levels (IgY) in ducks receiving the vaccines were higher than those in single gene delivered groups. Additionally, the copy number of covalently closed circular DNA in hepatocytes after DHBV challenge also provided evidence that our fusion vaccines could enhance the protective efficiency against DHBV infection in ducks.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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