TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell
Morgane Auboiron,
Pauline Vasseur,
Saphia Tonazzini,
Arame Fall,
Francesc Rubert Castro,
Iva Sučec,
Khadija El Koulali,
Serge Urbach,
Marta Radman-Livaja
Affiliations
Morgane Auboiron
Institut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France; Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France
Pauline Vasseur
Institut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France
Saphia Tonazzini
Institut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France; Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France
Arame Fall
Institut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France; Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France
Francesc Rubert Castro
Institut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France
Iva Sučec
Institut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France
Khadija El Koulali
Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France; Functional Proteomics Platform, IGF _ CNRS INSERM, Université de Montpellier, 141 rue de la Cardonille, 34094 Montpellier cedex 5, France
Serge Urbach
Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France; Functional Proteomics Platform, IGF _ CNRS INSERM, Université de Montpellier, 141 rue de la Cardonille, 34094 Montpellier cedex 5, France
Marta Radman-Livaja
Institut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France; Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France; Corresponding author
Summary: Inheritance of chromatin-bound proteins theoretically plays a role in the epigenetic transmission of cellular phenotypes. Protein segregation during cell division is however poorly understood. We now describe TrIPP (Tracking the Inheritance Patterns of Proteins): a live cell imaging method for tracking maternal proteins during asymmetric cell divisions of budding yeast. Our analysis of the partitioning pattern of a test set of 18 chromatin-associated proteins reveals that abundant and moderately abundant maternal proteins segregate stochastically and symmetrically between the two cells with the exception of Rxt3p, Fpr4p, and Tup1p, which are preferentially retained in the mother. Low abundance proteins also tend to be retained in the mother cell with the exception of Sir2p and the linker histone H1. Our analysis of chromatin protein behavior in single cells reveals potentially general trends such as coupled protein synthesis and decay and a correlation between protein half-lives and cell-cycle duration.
