Nature Communications (Mar 2021)
CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer
- Musaddeque Ahmed,
- Fraser Soares,
- Ji-Han Xia,
- Yue Yang,
- Jing Li,
- Haiyang Guo,
- Peiran Su,
- Yijun Tian,
- Hyung Joo Lee,
- Miranda Wang,
- Nayeema Akhtar,
- Kathleen E. Houlahan,
- Almudena Bosch,
- Stanley Zhou,
- Parisa Mazrooei,
- Junjie T. Hua,
- Sujun Chen,
- Jessica Petricca,
- Yong Zeng,
- Alastair Davies,
- Michael Fraser,
- David A. Quigley,
- Felix Y. Feng,
- Paul C. Boutros,
- Mathieu Lupien,
- Amina Zoubeidi,
- Liang Wang,
- Martin J. Walsh,
- Ting Wang,
- Shancheng Ren,
- Gong-Hong Wei,
- Housheng Hansen He
Affiliations
- Musaddeque Ahmed
- Princess Margaret Cancer Center/University Health Network
- Fraser Soares
- Princess Margaret Cancer Center/University Health Network
- Ji-Han Xia
- Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of Oulu
- Yue Yang
- Changhai Hospital
- Jing Li
- Changhai Hospital
- Haiyang Guo
- Princess Margaret Cancer Center/University Health Network
- Peiran Su
- Princess Margaret Cancer Center/University Health Network
- Yijun Tian
- Department of Tumor Biology, H. Lee Moffitt Cancer Center and Research Institute
- Hyung Joo Lee
- Department of Genetics, Washington University in St. Louis
- Miranda Wang
- Princess Margaret Cancer Center/University Health Network
- Nayeema Akhtar
- Princess Margaret Cancer Center/University Health Network
- Kathleen E. Houlahan
- Department of Medical Biophysics, University of Toronto
- Almudena Bosch
- Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai
- Stanley Zhou
- Princess Margaret Cancer Center/University Health Network
- Parisa Mazrooei
- Princess Margaret Cancer Center/University Health Network
- Junjie T. Hua
- Princess Margaret Cancer Center/University Health Network
- Sujun Chen
- Princess Margaret Cancer Center/University Health Network
- Jessica Petricca
- Princess Margaret Cancer Center/University Health Network
- Yong Zeng
- Princess Margaret Cancer Center/University Health Network
- Alastair Davies
- The Vancouver Prostate Centre, Vancouver General Hospital and Department of Urologic Sciences, The University of British Columbia
- Michael Fraser
- Princess Margaret Cancer Center/University Health Network
- David A. Quigley
- Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco
- Felix Y. Feng
- Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco
- Paul C. Boutros
- Vector Institute
- Mathieu Lupien
- Princess Margaret Cancer Center/University Health Network
- Amina Zoubeidi
- The Vancouver Prostate Centre, Vancouver General Hospital and Department of Urologic Sciences, The University of British Columbia
- Liang Wang
- Department of Tumor Biology, H. Lee Moffitt Cancer Center and Research Institute
- Martin J. Walsh
- Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai
- Ting Wang
- Department of Genetics, Washington University in St. Louis
- Shancheng Ren
- Changhai Hospital
- Gong-Hong Wei
- Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of Oulu
- Housheng Hansen He
- Princess Margaret Cancer Center/University Health Network
- DOI
- https://doi.org/10.1038/s41467-021-21867-0
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 15
Abstract
Prostate cancer risk-associated SNPs are enriched in noncoding CREs. Here the authors perform CRISPRi screens of CREs in prostate cancer cell lines to describe a causal mechanism synergistically driven by a risk SNP and DNA methylation-mediated 3D genome architecture.
