Single-cell technologies for multimodal omics measurements

Abstract

Read online

The recent surge in single-cell genomics, including the development of a wide range of experimental and computational approaches, has provided insights into the complex molecular networks of cells during development and in human diseases at unprecedented resolution. Single-cell transcriptome analysis has enabled high-resolution investigation of cellular heterogeneity in a wide range of cell populations ranging from early embryos to complex tissues—while posing the risk of only capturing a partial picture of the cells’ complex molecular networks. Single-cell multiomics technologies aim to bridge this gap by providing a more holistic view of the cell by simultaneously measuring multiple molecular types from the same cell and providing a more complete view of the interactions and combined functions of multiple regulatory layers at cell-type resolution. In this review, we briefly summarized the recent advances in multimodal single-cell technologies and discussed the challenges and opportunities of the field.

Keywords

• single-cell
• multi-omics
• genomics
• chromatin states
• transcriptome