Human induced pluripotent stem cell line with cytochrome P450 enzyme polymorphism (CYP2C19*2/CYP3A5*3C) generated from lymphoblastoid cells

Jaehun Lee; Dong-Hun Woo; Han-Jin Park; Kijung Park; Duck Sung Ko; Jong-Hoon Kim

Stem Cell Research (Mar 2018)

Human induced pluripotent stem cell line with cytochrome P450 enzyme polymorphism (CYP2C192/CYP3A53C) generated from lymphoblastoid cells

Jaehun Lee,
Dong-Hun Woo,
Han-Jin Park,
Kijung Park,
Duck Sung Ko,
Jong-Hoon Kim

Affiliations

Jaehun Lee: Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Sciences and Biotechnology, Science Campus, Korea University, Seoul, Republic of Korea
Dong-Hun Woo: Laboratory of Stem Cells, NEXEL Co., Ltd., Seoul, Republic of Korea
Han-Jin Park: Predictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of Korea
Kijung Park: Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Sciences and Biotechnology, Science Campus, Korea University, Seoul, Republic of Korea
Duck Sung Ko: Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Sciences and Biotechnology, Science Campus, Korea University, Seoul, Republic of Korea
Jong-Hoon Kim: Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Sciences and Biotechnology, Science Campus, Korea University, Seoul, Republic of Korea; Corresponding author.

Journal volume & issue: Vol. 27
pp. 34 – 37

Abstract

Read online

Cytochrome P450 (CYP) comprises a superfamily of monooxygenase responsible for the metabolism of xenobiotics and approximately 75% of drugs in use today. Thus, genetic polymorphisms in CYP genes contribute to interindividual differences in hepatic metabolism of drugs, affecting on individual drug efficacy and may cause adverse effects. Here, we generated a human induced pluripotent stem cell (hiPSC) line with pharmacologically important traits (CYP2C19*2/CYP3A5*3C), which are highly polymorphic in Asian from lymphoblastoid cells. This hiPSC line could be a valuable source for predicting individual drug responses in the drug screening process that uses hiPSC-derived somatic cells, including hepatocytes.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal