CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants
Xiaohui Zhan,
Fengjuan Zhang,
Ning Li,
Kai Xu,
Xiaodi Wang,
Shenghua Gao,
Yanxu Yin,
Weiling Yuan,
Weifang Chen,
Zhiyong Ren,
Minghua Yao,
Fei Wang
Affiliations
Xiaohui Zhan
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Fengjuan Zhang
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China
Ning Li
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Kai Xu
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Xiaodi Wang
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Shenghua Gao
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Yanxu Yin
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Weiling Yuan
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Weifang Chen
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Zhiyong Ren
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Minghua Yao
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Fei Wang
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430062, China
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas have been recognized as powerful genome-editing tools in diverse eukaryotic species, including plants, and thus hold great promise for engineering virus resistance in plants. Nevertheless, further attention is required regarding various issues associated with applying new powerful technologies in the field. This mini-review focuses on the recent advances in using CRISPR/Cas9 and CRISPR/Cas13 systems to combat DNA and RNA viruses in plants. We explored the utility of CRISPR/Cas for targeting the viral genome and editing host susceptibility genes in plants. We also provide insights into the limitations and challenges of using CRISPR/Cas for plant virus interference and propose individual combinatorial solutions. In conclusion, CRISPR/Cas technology has the potential to offer innovative and highly efficient approaches for controlling viruses in important crops in the near future.
