Evaluation of Anticancer Effect of Aqueous and Hydroalcoholic Extracts of Quercusin Fectoria Leaf against Colon Cancer HT29 Cell Line

Abstract

Background and Aim: In traditional medicine and previous studies, Quercus infectoria plants have been suggested as a cancer treatment. The aim of the current study was to investigate the cytotoxic effect of aqueous and hydro alcoholic extracts of Quercus infectoria leaf against colon cancer HT29 cell line and to evaluate the Bax and Bcl-2 gene expression in treated cells. Materials and Methods: In this study, aqueoFus and hydro alcoholic extracts of Quercus infectoria leaf were prepared. Then, the HT29 and HEK293 cell lines were treated by various concentrations of extracts for 24 hours and the cytotoxicity effect of extracts was estimated by colorimetric MTT (methyl thiazolyl tetrazolium) assay. Finally, the pro-apoptotic Bax and anti-apoptotic Bcl-2 gene expression in treated cells compared to GAPDH reference gene expression was evaluated using real time PCR technique. Findings: According to the MTT results, the cytotoxic activity of aqueous extract has dose-dependent manner against both cell lines, therefore, the Bax and Bcl-2 gene expression levels in treated cells by aqueous extract were changed 2.8 (p˂0.05) and 2.2-fold (p˃0.05) compared to reference gene, respectively. Conclusion: According to the results, it seems that the aqueous extract of Quercus infectoria leaf has the potential for apoptosis induction in colon cancer HT29 cell line and based on more studies, it can be used as a colon cancer treatment.

Keywords

• Quercus infectoria
• Bax
• Bcl-2
• Apoptosi
• Colon cancer