Carbon Sources as a Factor Determining the Activity of Microbial Oxidation of Sulfide Concentrate at Elevated Temperature

Abstract

The goal of the present work was to evaluate the possibility of improving the efficiency of the stirred tank reactor biooxidation of sulfide gold-bearing concentrate by means of addition of carbon sources required for the constructive metabolism of microorganisms. Biooxidation experiments were performed on gold-bearing pyrite-arsenopyrite concentrate in continuous mode at 45 °C to determine the influence of additional carbon sources (carbon dioxide and molasses) on sulfide mineral oxidation. The use of CO2 allowed increasing the efficiency of the biooxidation and the extents of sulfide sulfur (Ss) oxidation and gold recovery were 79% and 84%, respectively. Biooxidation in a control experiment (without additional carbon sources) and when using molasses allowed achieving 39% and 66% oxidation of Ss as well as 73% and 81% of gold recovery. Analysis of the microbial populations formed in biooxidation reactors using NGS methods demonstrated that CO2 application led to an increase in the relative abundance of the genus Sulfobacillus. Thus, it was determined that application of additional carbon source makes it possible to manage the biooxidation process, affecting both sulfide mineral oxidation and microbial population composition.

Keywords

• biohydrometallurgy
• acidophilic microorganisms
• pyrite-arsenopyrite gold-bearing sulfide concentrates
• carbon dioxide
• molasses