Cry1Ab protein quantification in leaves, stems and grains, and effectiveness to control Spodoptera frugiperda and Helicoverpa zea on two hybrids of genetically modified corn

Abstract

A study was carried out to evaluate the infestation and associated damages to the presence of the Spodoptera frugiperda and Helicoverpa zea caterpillars, in two genetically modified (GM) corn, Dekalb DKB390 and Agroceres AG8088, expressing the cry1Ab protein. For this objective, an split-splot design with two factors (hybrid x gene) was carried out. Negative controls were made with the same corn hybrids without the gene cry1Ab (NoGM). The concentration of the protein Cry1Ab was determined by the ELISA (enzyme linked immuno sorbent assay) technique in previously dehydrated stems, leaves and grains of GM corns. Caterpillars sampling of S. frugiperda and associated damage survey were accomplished at 15, 22, 29, 36 and 42 days after the sowing, according to a damage scale with 5 levels (0- pest absence to 5- dead plant). Countings of H. zea caterpillars and associated damage were assessed at 57, 71, 78 and 85 days after the sowing, according to a damage scale with 4 levels (0-pest absence curse to 4-gallery in the corn cob minor than 3cm). Sampled caterpillars were divided in two groups, smaller or equal to 15mm and bigger than 15mm. No insecticide application was accomplished in the GM blocks while NoGM blocks were sprayed with deltametrina (2,8%), 42 days after the sowing. The infestation level and associated damage due to S. frugiperda presence was significantly smaller (p < 0,05) in the GM corns in comparison to NoGM corns. Nevertheless, the number and associated damage of S. frugiperda caterpillars, smaller than 15 mm, were superior in the GM DKB390 corn when compared to the GM AG8088 corn. Differences were not observed in the S. frugiperda infestation and associated damage between GM corns and between NoGM corns. On average, the concentration of Cry1Ab protein was significantly superior in leaves and stems in comparison to the grain and, usually, superior in the GM AG8088 corn comparatively to GM DKB390 corn. No differences were found on level damages caused by caterpillars of H. zea in GM and NoGM AG8088 corns, after 71, 78 and 85 days of sowing. In conclusion, GM corns were more efficient in the limitation of S. frugiperda in comparison with H. zea, a result that can be associated to the largest Cry1Ab protein concentration observed in leafs (8.062 ug/g fresh tissue) and stems (5.626 ug/g fresh tissue) in comparison to the existent in the grain (0.372 ug/g fresh tissue). Also, ELISA technique proved to be very efficient in Cry1Ab protein quantification on corn.

Keywords

• Milhos transgénicos
• proteina Cry1Ab
• Spodoptera frugiperda
• Helicoverpa zea