Nature Communications (Aug 2019)
An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters
- Tingting Hao,
- Zhoujie Xie,
- Min Wang,
- Liwei Liu,
- Yuwei Zhang,
- Weicang Wang,
- Zhao Zhang,
- Xuejin Zhao,
- Pengwei Li,
- Zhengyan Guo,
- Shushan Gao,
- Chunbo Lou,
- Guodong Zhang,
- Justin Merritt,
- Geoff P. Horsman,
- Yihua Chen
Affiliations
- Tingting Hao
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Zhoujie Xie
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Min Wang
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Liwei Liu
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Yuwei Zhang
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Weicang Wang
- Department of Food Science, University of Massachusetts
- Zhao Zhang
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Xuejin Zhao
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Pengwei Li
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Zhengyan Guo
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Shushan Gao
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Chunbo Lou
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- Guodong Zhang
- Department of Food Science, University of Massachusetts
- Justin Merritt
- Department of Restorative Dentistry, Oregon Health & Science University
- Geoff P. Horsman
- Department of Chemistry and Biochemistry, Wilfrid Laurier University
- Yihua Chen
- State Key Laboratory of Microbial Resources, and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences
- DOI
- https://doi.org/10.1038/s41467-019-11673-0
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 13
Abstract
Anaerobic bacteria represent a rich source of biological and chemical diversity but are difficult to cultivate and there is a lack of heterologous expression systems. Here the authors develop an expression system based on S. mutans UA159 for biosynthetic gene clusters from anaerobic bacteria.
