VISTA antibody-loaded Fe3O4@TiO2 nanoparticles for sonodynamic therapy-synergistic immune checkpoint therapy of pancreatic cancer

Lu Hong; Kaiwei Xu; Ming Yang; Lubing Zhu; Chunqu Chen; Liu Xu; Weihao Zhu; Lufei Jin; Linwei Wang; Jie Lin; Jianhua Wang; Wenzhi Ren; Aiguo Wu

Materials Today Bio (Jun 2024)

VISTA antibody-loaded Fe3O4@TiO2 nanoparticles for sonodynamic therapy-synergistic immune checkpoint therapy of pancreatic cancer

Lu Hong,
Kaiwei Xu,
Ming Yang,
Lubing Zhu,
Chunqu Chen,
Liu Xu,
Weihao Zhu,
Lufei Jin,
Linwei Wang,
Jie Lin,
Jianhua Wang,
Wenzhi Ren,
Aiguo Wu

Affiliations

Lu Hong: The First Affiliated Hospital of Ningbo University, Ningbo, 315010, PR China; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China
Kaiwei Xu: The First Affiliated Hospital of Ningbo University, Ningbo, 315010, PR China; Health Science Center, Ningbo University, Ningbo, 315210, PR China
Ming Yang: Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China
Lubing Zhu: The First Affiliated Hospital of Ningbo University, Ningbo, 315010, PR China; Health Science Center, Ningbo University, Ningbo, 315210, PR China
Chunqu Chen: The First Affiliated Hospital of Ningbo University, Ningbo, 315010, PR China; Health Science Center, Ningbo University, Ningbo, 315210, PR China
Liu Xu: The First Affiliated Hospital of Ningbo University, Ningbo, 315010, PR China
Weihao Zhu: Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China
Lufei Jin: The First Affiliated Hospital of Ningbo University, Ningbo, 315010, PR China
Linwei Wang: The First Affiliated Hospital of Ningbo University, Ningbo, 315010, PR China; Health Science Center, Ningbo University, Ningbo, 315210, PR China
Jie Lin: Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Advanced Energy Science and Technology Guangdong Laboratory, Huizhou, 516000, PR China
Jianhua Wang: The First Affiliated Hospital of Ningbo University, Ningbo, 315010, PR China; Health Science Center, Ningbo University, Ningbo, 315210, PR China; Corresponding author. Ningbo University Affiliated First Hospital, Ningbo, 315010, PR China.
Wenzhi Ren: Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Advanced Energy Science and Technology Guangdong Laboratory, Huizhou, 516000, PR China; Corresponding author. Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China.
Aiguo Wu: Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Advanced Energy Science and Technology Guangdong Laboratory, Huizhou, 516000, PR China; Corresponding author. Advanced Energy Science and Technology Guangdong Laboratory, Huizhou, 516000, PR China.

Journal volume & issue: Vol. 26
p. 101106

Abstract

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Breaking the poor permeability of immune checkpoint inhibitors (ICIs) caused by the stromal barrier and reversing the immunosuppressive microenvironment are significant challenges in pancreatic cancer immunotherapy. In this study, we synthesized core-shell Fe3O4@TiO2 nanoparticles to act as carriers for loading VISTA monoclonal antibodies to form Fe3O4@TiO2@VISTAmAb (FTV). The nanoparticles are designed to target the overexpressed ICIs VISTA in pancreatic cancer, aiming to improve magnetic resonance imaging-guided sonodynamic therapy (SDT)-facilitated immunotherapy. Laser confocal microscopy and flow cytometry results demonstrate that FTV nanoparticles are specifically recognized and phagocytosed by Panc-2 cells. In vivo experiments reveal that ultrasound-triggered TiO2 SDT can induce tumor immunogenic cell death (ICD) and recruit T-cell infiltration within the tumor microenvironment by releasing damage-associated molecular patterns (DAMPs). Furthermore, ultrasound loosens the dense fibrous stroma surrounding the pancreatic tumor and increases vascular density, facilitating immune therapeutic efficiency. In summary, our study demonstrates that FTV nanoparticles hold great promise for synergistic SDT and immunotherapy in pancreatic cancer.

Published in Materials Today Bio

ISSN: 2590-0064 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: https://www.journals.elsevier.com/materials-today-bio

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