Capture and storage of plant genomic DNA on a readily available cellulose matrix

Abstract

Whatman FTA® Cards are a fast and efficient method for capturing and storing nucleic acids but can be cost-prohibitive for large numbers of samples. We developed a method that substitutes a readily-available cellulose matrix and homemade washing buffer for commercial FTA® Cards and FTA® Purification Reagent. This method is suitable for long-term storage of DNA from many plant species prior to PCR.

Keywords

• DNA
• FTA® Cards
• FTA purification reagent
• PCR
• polymerase chain reaction