MiR-145 is involved in the proliferation of bovine mammary epithelial cells and regulates bovine insulin receptor substrate 1

Abstract

MicroRNAs (miRNAs) are short noncoding RNAs that can regulate target gene expression at the posttranscriptional level. It has been reported that bta-miR-145 shows differential expression between the different lactation stages of bovine mammary glands, but the function of bta-miR-145 in normal mammary glands remains unknown. The overexpression of bta-miR-145 significantly decreased the proliferation of mammary epithelial cells (p .05), whereas the IRS1 protein level was changed significantly after bta-miR-145 mimic or inhibitor transfection for 72 h (p < .05). Taken together, these results suggest that bta-miR-145 is involved in the proliferation of bovine mammary epithelial cells by targeting IRS1, which is related to the MAPK signalling pathway.Highlights Bta-miR-145 affects bovine mammary epithelial cell vitality in primary bovine mammary epithelial cells. IRS1 was verified as target gene of bta-miR-145. IRS1 belongs to MAPK signaling pathway, which regulate cell proliferation.

Keywords

• bta-mir-145
• irs1
• primary bovine mammary epithelial cell