Lactobacillus rhamnosus Strains Relieve Loperamide-Induced Constipation via Different Pathways Independent of Short-Chain Fatty Acids

Gang Wang; Gang Wang; Shurong Yang; Shurong Yang; Shanshan Sun; Shanshan Sun; Qian Si; Qian Si; Linlin Wang; Linlin Wang; Qiuxiang Zhang; Qiuxiang Zhang; Gaojue Wu; Jianxin Zhao; Jianxin Zhao; Jianxin Zhao; Jianxin Zhao; Hao Zhang; Hao Zhang; Hao Zhang; Hao Zhang; Hao Zhang; Wei Chen; Wei Chen; Wei Chen; Wei Chen

doi:10.3389/fcimb.2020.00423

Frontiers in Cellular and Infection Microbiology (Aug 2020)

Lactobacillus rhamnosus Strains Relieve Loperamide-Induced Constipation via Different Pathways Independent of Short-Chain Fatty Acids

Gang Wang,
Gang Wang,
Shurong Yang,
Shurong Yang,
Shanshan Sun,
Shanshan Sun,
Qian Si,
Qian Si,
Linlin Wang,
Linlin Wang,
Qiuxiang Zhang,
Qiuxiang Zhang,
Gaojue Wu,
Jianxin Zhao,
Jianxin Zhao,
Jianxin Zhao,
Jianxin Zhao,
Hao Zhang,
Hao Zhang,
Hao Zhang,
Hao Zhang,
Hao Zhang,
Wei Chen,
Wei Chen,
Wei Chen,
Wei Chen

Affiliations

Gang Wang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Gang Wang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Shurong Yang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Shurong Yang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Shanshan Sun: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Shanshan Sun: School of Food Science and Technology, Jiangnan University, Wuxi, China
Qian Si: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Qian Si: School of Food Science and Technology, Jiangnan University, Wuxi, China
Linlin Wang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Linlin Wang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Qiuxiang Zhang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Qiuxiang Zhang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Gaojue Wu: Department of Gastroenterology, The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, Wuxi, China
Jianxin Zhao: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Jianxin Zhao: School of Food Science and Technology, Jiangnan University, Wuxi, China
Jianxin Zhao: International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi, China
Jianxin Zhao: (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, China
Hao Zhang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Hao Zhang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Hao Zhang: (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, China
Hao Zhang: National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, China
Hao Zhang: Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi, China
Wei Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Wei Chen: School of Food Science and Technology, Jiangnan University, Wuxi, China
Wei Chen: National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, China
Wei Chen: Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, China

DOI: https://doi.org/10.3389/fcimb.2020.00423
Journal volume & issue: Vol. 10

Abstract

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Increasing researches have confirmed the relationship between slow-transit constipation and gut microbiota dysbiosis. Many population and animal experiments have identified probiotics as effectors for the relief of constipation symptoms, but the specific mechanism remains unclear. In this intervention study, Lactobacillus rhamnosus strains isolated from five different sources were administered to mice with loperamide-induced constipation, and the impacts of these strains on constipation-related indicators were evaluated. All five strains of L. rhamnosus were found to improve constipation to various degrees. However, contrary to previous studies, the abilities of L. rhamnosus strains to improve constipation symptoms were not associated with the levels of short-chain fatty acids (SCFAs) in the colon. The effects of different strains of L. rhamnosus on constipation relief were associated with different aspects of the GI tract, including gastrointestinal regulatory peptides, neurotransmitters, neurotrophic factors, and gut microbiota. The findings of this study demonstrate that L. rhamnosus strains can alleviate constipation-related symptoms via different pathways independent of SCFAs regulation. This study yields a new perspective for clinical use of probiotics to better improve constipation symptoms, by combining strains with different mechanisms for alleviation of constipation.

Published in Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/Cellular-and-Infection-Microbiology

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