Methotrexate and Cytarabine—Loaded Nanocarriers for Multidrug Cancer Therapy. Spectroscopic Study

Danuta Pentak; Violetta Kozik; Andrzej Bąk; Paulina Dybał; Aleksander Sochanik; Josef Jampilek

doi:10.3390/molecules21121689

Molecules (Dec 2016)

Methotrexate and Cytarabine—Loaded Nanocarriers for Multidrug Cancer Therapy. Spectroscopic Study

Danuta Pentak,
Violetta Kozik,
Andrzej Bąk,
Paulina Dybał,
Aleksander Sochanik,
Josef Jampilek

Affiliations

Danuta Pentak: Department of Materials Chemistry and Chemical Technology, Institute of Chemistry, University of Silesia, 40-006 Katowice, Poland
Violetta Kozik: Department of Synthesis Chemistry, Institute of Chemistry, University of Silesia, 40-006 Katowice, Poland
Andrzej Bąk: Department of Organic Chemistry, Institute of Chemistry, University of Silesia, 40-006 Katowice, Poland
Paulina Dybał: Department of Organic Chemistry, Institute of Chemistry, University of Silesia, 40-006 Katowice, Poland
Aleksander Sochanik: Center for Translational Research and Molecular Biology of Cancer, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, 44-100 Gliwice, Poland
Josef Jampilek: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, Slovakia

DOI: https://doi.org/10.3390/molecules21121689
Journal volume & issue: Vol. 21, no. 12
p. 1689

Abstract

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Determining the properties of nanoparticles obtained by novel methods and defining the scope of their application as drug carriers has important practical significance. This article presents the pioneering studies concerning high degree incorporation of cytarabine (AraC) and methotrexate (MTX) into liposome vesicles. The main focus of this study were cytarabine-methotrexate-dipalmitoylphosphatidylcholine (DPPC) interactions observed in the gel and fluid phases of DPPC bilayers. The proposed new method of use the Transmittance2919/2850 ratio presented in our research is sensitive to subtle changes in conformational order resulting from rotations, kinks and bends of the lipid chains. The transition temperatures characterized by Fourier Transform Infrared Spectroscopy (FT-IR) were consistent with the results obtained by Differential Scanning Calorimetry (DSC). Transmission Electron Microscopy (TEM) was used in order to determine the size and shape of the liposomes obtained. The mutual interactions occurring between the drugs studied and the phospholipids were analyzed using the Nuclear Magnetic Resonance (NMR).

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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