Rhamnetin abrogates polystyrene microplastics prompted hepatic damage by regulating Nrf-2/Keap-1 pathway

Abstract

Polystyrene microplastics (PS-MPs) are environmental toxicants that exert adverse effects on organisms. Rhamnetin (RHM) is a natural flavone that shows multiple therapeutic potentials. Therefore, the present study was planned to determine the mitigative effect of RHM against PS-MPs induced liver damage. 48 rats were divided into 4 groups. Control group, PS-MPs (0.01 mg/kg) administered group, PS-MPs (0.01 mg/kg)+ RHM (50 mg/kg) co-administered group and RHM alone (50 mg/kg) administered group. PS-MPs reduced the expressions of Nrf-2 and anti-oxidant gene expressions, while increasing Keap-1 expression. PS-MPs also decreased the activities of catalase (CAT), glutathione reductase (GSR), heme oxygenase-1 (HO-1), glutathione-S-transferase (GST), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione (GSH), besides elevating the levels of MDA and ROS. Additionally, PS-MPs augmented the levels of alanine transaminase (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST). Furthermore, there was an upsurge in the levels of inflammatory indices in PS-MPs treated group. PS-MPs intoxication also increased Bax and Caspase-3 expressions, while lowering the Bcl-2 expression. Nevertheless, RHM mitigated all the damages due to its hepatoprotective, anti-inflammatory, anti-oxidant and anti-apoptotic potentials.

Keywords

• Hepatotoxicity
• Rhamnetin
• Polystyrene microplastics
• Apoptosis
• Oxidative stress