STUDY OF APOPTOSIS OF IMMUNE CELLS IN TYPE 1 DIABETES MELLITUS

Abstract

Type 1 diabetic mellitus (T1DM) is known to be associated with progressive destruction of β-cells of the pancreas. Dysregulated immunity and programmed cell death are an important link in pathogenesis of diabetes. In this study, we examined expression levels of interleukin-2, Bcl-1, ANXA-11 genes in patients with T1DM. The study was done with blood leukocures of T1DM patients (30) and healthy controls (70). Reverse-transcription PCR was done with Transcriptor First Strand cDNA Synthesis Kit from Roche Life Science. The study of IL-2 and Bcl-1 gene expression level in peripheral blood leukocytes indicated that the median gene expression levels of IL-2 and Bcl-1 are increased in patients with T1DM patients compared with control group of healthy persons. The study of apoptosis by annexin test has revealed an increased level of ANXA 11 expression in T1DM patients. The obtained data can serve as an additional source for understanding the pathogenesis of T1DM mechanisms.

Keywords

• diabetes
• leukocytes
• interleukin-2
• annexin
• proliferation
• apoptosis