Isolation of mesenchymal stem cells from fetal tissues

Abstract

Backgrond&Objective: The ability of mesenchymalstem cells (MSCs) differentiation into many cell types, as well as their ex vivo expansion potential, makes them as an attractive therapeutic tool for cell transplantation and tissue engineering. This project was designed to improve isolation culture and characterization of human amniotic membran-derivedMSSCS and human warton ,s jelly- derived MSCs. Materials and Methods: The amniotic membrane and warton ,s jelly fragments were cultured as explants in the culture plates. After fourth passage, a population of homogenous cells was isolated from human amniotic membrane and warton 'sjelly. The phenotype identification of adherent cell was performed by flowcytometric analysis. The in vitro differentiation of MSSCS into osteoblast and adipocytes was also achieved. Resalts: The differentiation of osteoblasts was determined by deposition of a mineralized extracellular matrix in the culture plates that detected with Alizarin Red. A dipocyts were easily identified by their morphology and staining with Oil Red.AM-HMSSCS and the UC-HMSSCS both showed thanCD105, CD44positiveexpression and thanCD34, CD133 negative expression. In addition, the differentiation of specific staining, indicative of mesenchymal stem cells differentiating into precursor cells of bone precursor cells of adipose tissue. Conclusion: It seems that both samples are similar and suitable for cell therapy.

Keywords

• Mesenchymal Stem Cells
• Amniotic Membran
• Warton's Jelly.