Frontiers in Immunology (Sep 2020)
Longitudinal Analysis of T and B Cell Receptor Repertoire Transcripts Reveal Dynamic Immune Response in COVID-19 Patients
- Xuefeng Niu,
- Song Li,
- Song Li,
- Pingchao Li,
- Wenjing Pan,
- Wenjing Pan,
- Qian Wang,
- Ying Feng,
- Xiaoneng Mo,
- Qihong Yan,
- Xianmiao Ye,
- Jia Luo,
- Linbing Qu,
- Daniel Weber,
- Miranda L. Byrne-Steele,
- Zhe Wang,
- Fengjia Yu,
- Fang Li,
- Richard M. Myers,
- Michael T. Lotze,
- Nanshan Zhong,
- Jian Han,
- Jian Han,
- Jian Han,
- Ling Chen,
- Ling Chen,
- Ling Chen
Affiliations
- Xuefeng Niu
- State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
- Song Li
- Jiangsu Industrial Technology Research Institute (JITRI), Applied Adaptome Immunology Institute, Nanjing, China
- Song Li
- iRepertoire Inc., Huntsville, AL, United States
- Pingchao Li
- Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangdong Laboratory of Computational Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
- Wenjing Pan
- iRepertoire Inc., Huntsville, AL, United States
- Wenjing Pan
- HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States
- Qian Wang
- Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangdong Laboratory of Computational Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
- Ying Feng
- State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
- Xiaoneng Mo
- Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou, China
- Qihong Yan
- Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangdong Laboratory of Computational Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
- Xianmiao Ye
- Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangdong Laboratory of Computational Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
- Jia Luo
- Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangdong Laboratory of Computational Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
- Linbing Qu
- Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangdong Laboratory of Computational Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
- Daniel Weber
- iRepertoire Inc., Huntsville, AL, United States
- Miranda L. Byrne-Steele
- iRepertoire Inc., Huntsville, AL, United States
- Zhe Wang
- Jiangsu Industrial Technology Research Institute (JITRI), Applied Adaptome Immunology Institute, Nanjing, China
- Fengjia Yu
- Jiangsu Industrial Technology Research Institute (JITRI), Applied Adaptome Immunology Institute, Nanjing, China
- Fang Li
- Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou, China
- Richard M. Myers
- HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States
- Michael T. Lotze
- Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, United States
- Nanshan Zhong
- State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
- Jian Han
- Jiangsu Industrial Technology Research Institute (JITRI), Applied Adaptome Immunology Institute, Nanjing, China
- Jian Han
- iRepertoire Inc., Huntsville, AL, United States
- Jian Han
- HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States
- Ling Chen
- State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
- Ling Chen
- Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangdong Laboratory of Computational Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
- Ling Chen
- Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou, China
- DOI
- https://doi.org/10.3389/fimmu.2020.582010
- Journal volume & issue
-
Vol. 11
Abstract
Severe COVID-19 is associated with profound lymphopenia and an elevated neutrophil to lymphocyte ratio. We applied a novel dimer avoidance multiplexed polymerase chain reaction next-generation sequencing assay to analyze T (TCR) and B cell receptor (BCR) repertoires. Surprisingly, TCR repertoires were markedly diminished during the early onset of severe disease but recovered during the convalescent stage. Monitoring TCR repertoires could serve as an indicative biomarker to predict disease progression and recovery. Panoramic concurrent assessment of BCR repertoires demonstrated isotype switching and a transient but dramatic early IgA expansion. Dominant B cell clonal expansion with decreased diversity occurred following recovery from infection. Profound changes in T cell homeostasis raise critical questions about the early events in COVID-19 infection and demonstrate that immune repertoire analysis is a promising method for evaluating emergent host immunity to SARS-CoV-2 viral infection, with great implications for assessing vaccination and other immunological therapies.
Keywords
