Journal of Venomous Animals and Toxins including Tropical Diseases (Jan 2010)

Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens

  • VB Richini-Pereira,
  • SMG Bosco,
  • RC Theodoro,
  • L Barrozo,
  • E Bagagli

DOI
https://doi.org/10.1590/S1678-91992010000400011
Journal volume & issue
Vol. 16, no. 4
pp. 607 – 613

Abstract

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Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several fungi, as well as determining natural reservoirs in wild animals and facilitating the understanding of host-pathogen relationships.

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