Understanding the molecular pathway of triclosan-induced ADHD-like behaviour: Involvement of the hnRNPA1-PKM2-STAT3 feedback loop

Chang Shu; He Cui; Yuxuan Peng; Ziyun Wei; Xiao Ni; Linlin Zheng; Jianing Shang; Fu Liu; Jieyu Liu

Environment International (Sep 2024)

Understanding the molecular pathway of triclosan-induced ADHD-like behaviour: Involvement of the hnRNPA1-PKM2-STAT3 feedback loop

Chang Shu,
He Cui,
Yuxuan Peng,
Ziyun Wei,
Xiao Ni,
Linlin Zheng,
Jianing Shang,
Fu Liu,
Jieyu Liu

Affiliations

Chang Shu: Department of Health Laboratory Technology, School of Public Health, China Medical University, Shenyang 110122, PR China
He Cui: Department of Health Laboratory Technology, School of Public Health, China Medical University, Shenyang 110122, PR China
Yuxuan Peng: Department of Health Laboratory Technology, School of Public Health, China Medical University, Shenyang 110122, PR China
Ziyun Wei: Department of Health Laboratory Technology, School of Public Health, China Medical University, Shenyang 110122, PR China
Xiao Ni: Department of Health Laboratory Technology, School of Public Health, China Medical University, Shenyang 110122, PR China
Linlin Zheng: Department of Health Laboratory Technology, School of Public Health, China Medical University, Shenyang 110122, PR China
Jianing Shang: Department of Health Laboratory Technology, School of Public Health, China Medical University, Shenyang 110122, PR China
Fu Liu: Department of Health Laboratory Technology, School of Public Health, China Medical University, Shenyang 110122, PR China
Jieyu Liu: Key Laboratory of Environmental Stress and Chronic Disease Control & Prevention (China Medical University), Ministry of Education, PR China; Department of Health Laboratory Technology, School of Public Health, China Medical University, Shenyang 110122, PR China; Corresponding author at: Key Laboratory of Environmental Stress and Chronic Disease Control & Prevention (China Medical University), Ministry of Education, PR China.

Journal volume & issue: Vol. 191
p. 108966

Abstract

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Triclosan (TCS) is an environmental pollutant. In recent years, there has been increasing level of concern regarding the potential toxicity of TCS in animals and humans, especially its effects on the nervous system. However, whether TCS induces ADHD-like behaviour and the mechanism by which it affects neural function are unclear. The impact of 60 days of continuous exposure to TCS on the behaviour of offspring rats was assessed in this research. According to the results of this study, TCS exposure led to ADHD-like behaviour in offspring rats and activated microglia in the prefrontal cortex (PFC), inducing inflammatory factor release. In vitro studies showed that TCS increased the levels of inflammatory cytokines, including interleukin (IL)-1β, IL-6 and tumour necrosis factor (TNF)-α, in HMC3 cells. More importantly, we found that TCS regulated the STAT3 pathway by upregulating PKM2 via hnRNPA1. In summary, this study suggested that TCS can induce ADHD-like behaviour in offspring rats and continuously activate HMC3 microglia through the hnRNPA1-PKM2-STAT3 feedback loop, promoting inflammatory cytokine secretion.

Published in Environment International

ISSN: 0160-4120 (Print)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/environment-international

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