Stem Cell Research (Oct 2022)

Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

  • Ji-Young Kang,
  • Dasom Mun,
  • Yumin Chun,
  • Da-Seul Park,
  • Hyoeun Kim,
  • Nuri Yun,
  • Seung-Hyun Lee,
  • Boyoung Joung

Journal volume & issue
Vol. 64
p. 102901

Abstract

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TTN mutations are the common genetic cause for various types of cardiomyopathies (e.g., dilated cardiomyopathy, hypertrophic cardiomyopathy, restrictive cardiomyopathy, and arrhythmogenic right ventricular cardiomyopathy) and skeletal myopathies. Here, we generated three TTN knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 system. These cell lines, which exhibit normal karyotype, typical morphology and pluripotency, could provide useful platform for investigating the role of TTN in associated disorders.