A litmus test for classifying recognition mechanisms of transiently binding proteins

Kalyan S. Chakrabarti; Simon Olsson; Supriya Pratihar; Karin Giller; Kerstin Overkamp; Ko On Lee; Vytautas Gapsys; Kyoung-Seok Ryu; Bert L. de Groot; Frank Noé; Stefan Becker; Donghan Lee; Thomas R. Weikl; Christian Griesinger

doi:10.1038/s41467-022-31374-5

Nature Communications (Jul 2022)

A litmus test for classifying recognition mechanisms of transiently binding proteins

Kalyan S. Chakrabarti,
Simon Olsson,
Supriya Pratihar,
Karin Giller,
Kerstin Overkamp,
Ko On Lee,
Vytautas Gapsys,
Kyoung-Seok Ryu,
Bert L. de Groot,
Frank Noé,
Stefan Becker,
Donghan Lee,
Thomas R. Weikl,
Christian Griesinger

Affiliations

Kalyan S. Chakrabarti: Division of Sciences, Krea University
Simon Olsson: Department of Computer Science and Engineering, Chalmers University of Technology
Supriya Pratihar: Department of NMR Based Structural Biology, Max Planck Institute for Multidisciplinary Sciences
Karin Giller: Department of NMR Based Structural Biology, Max Planck Institute for Multidisciplinary Sciences
Kerstin Overkamp: Department of NMR Based Structural Biology, Max Planck Institute for Multidisciplinary Sciences
Ko On Lee: Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Korea Basic Science Institute, Ochang-Eup
Vytautas Gapsys: Department of Theoretical and Computational Biophysics, Max Planck Institute for Multidisciplinary Sciences
Kyoung-Seok Ryu: Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Korea Basic Science Institute, Ochang-Eup
Bert L. de Groot: Department of Theoretical and Computational Biophysics, Max Planck Institute for Multidisciplinary Sciences
Frank Noé: Department of Mathematics and Computer Science, Freie Universität Berlin
Stefan Becker: Department of NMR Based Structural Biology, Max Planck Institute for Multidisciplinary Sciences
Donghan Lee: Department of Medicine, James Graham Brown Cancer Center, University of Louisville
Thomas R. Weikl: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces
Christian Griesinger: Department of NMR Based Structural Biology, Max Planck Institute for Multidisciplinary Sciences

DOI: https://doi.org/10.1038/s41467-022-31374-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

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The authors provide a litmus test for the recognition mechanism of transiently binding proteins based on nuclear magnetic resonance and find a conformational selection binding mechanism through concentration-dependent kinetics of ubiquitin and SH3.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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