Identification of active chemical constituents of Asplenium ruprechtii Sa. Kurata based on in vitro neuroprotective activity evaluation

Zhi-Bo Jiang; Xing Lu; Jing-Zhi Chen; Xiao-Li Ma; Yi-Hu Ke; Xin Guo; Hai Liu; Chong-Long Li; Fang Wang; Xiu-Li Wu; Dai-Zhou Zhang; Shuang Cao

Arabian Journal of Chemistry (Dec 2020)

Identification of active chemical constituents of Asplenium ruprechtii Sa. Kurata based on in vitro neuroprotective activity evaluation

Zhi-Bo Jiang,
Xing Lu,
Jing-Zhi Chen,
Xiao-Li Ma,
Yi-Hu Ke,
Xin Guo,
Hai Liu,
Chong-Long Li,
Fang Wang,
Xiu-Li Wu,
Dai-Zhou Zhang,
Shuang Cao

Affiliations

Zhi-Bo Jiang: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China; Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, PR China
Xing Lu: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China
Jing-Zhi Chen: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China
Xiao-Li Ma: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China; Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, PR China
Yi-Hu Ke: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China; Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, PR China
Xin Guo: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China; Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, PR China
Hai Liu: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China; Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, PR China
Chong-Long Li: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China; Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, PR China; Corresponding authors at: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China (C.-L. Li). Institute of Traditional Chinese Medicine, Shandong Academy of Pharmaceutical Sciences, Jinan 250101, PR China (F. Wang).
Fang Wang: Shandong Academy of Pharmaceutical Sciences, Jinan 250101, PR China; Corresponding authors at: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China (C.-L. Li). Institute of Traditional Chinese Medicine, Shandong Academy of Pharmaceutical Sciences, Jinan 250101, PR China (F. Wang).
Xiu-Li Wu: College of Pharmacy, Ningxia Medical University, Yinchuan 750004, PR China; Corresponding authors at: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, PR China (C.-L. Li). Institute of Traditional Chinese Medicine, Shandong Academy of Pharmaceutical Sciences, Jinan 250101, PR China (F. Wang).
Dai-Zhou Zhang: Shandong Academy of Pharmaceutical Sciences, Jinan 250101, PR China
Shuang Cao: Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430072, PR China

Journal volume & issue: Vol. 13, no. 12
pp. 8815 – 8823

Abstract

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Mining structural novel and bioactive natural products from traditional Chinese drugs and folk medicines have attracted attention from pharmacologists and pharmaceutical chemists for a long time. In this study, the chemical constituents of Asplenium ruprechtii Sa. Kurata was systematically studied based on the evaluation of neuroprotective activity on hydrogen peroxide (H2O2)-induced damage of SH-SY5Y cell lines. Sixteen chemical monomers (1–16), including two new structures of 9,19-cycloartane-triterpenoid saponins (1,2), were discovered. Their chemical structures were established based on extensive spectroscopic data analysis, including the one- and two-dimensional (1D and 2D) nuclear magnetic resonance (NMR) data and high-resolution electrospray ionization mass spectrometry (HRESIMS). All monomer compounds were docked with an “active pocket” at the N-terminal of the GluN2B protein, where compounds 13–16 were found to possess high binding scores, which warrant further study on the molecular mechanism. Our findings provide insight and research techniques for discovering new drugs based on neuroprotective activity.

Published in Arabian Journal of Chemistry

ISSN: 1878-5352 (Print)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry
Website: http://www.journals.elsevier.com/arabian-journal-of-chemistry/

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