Methionine supplemented in a high fat-diet: Effects of growth performance and lipid metabolism in rice field eel (Monopterus albus)

Yajun Hu; Minglang Cai; Junzhi Zhang; Jihong Dai; Huan Zhong; Wuying Chu; Wencheng Xu; Yi Hu; Kaijian Chen

Aquaculture Reports (Oct 2023)

Methionine supplemented in a high fat-diet: Effects of growth performance and lipid metabolism in rice field eel (Monopterus albus)

Yajun Hu,
Minglang Cai,
Junzhi Zhang,
Jihong Dai,
Huan Zhong,
Wuying Chu,
Wencheng Xu,
Yi Hu,
Kaijian Chen

Affiliations

Yajun Hu: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
Minglang Cai: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
Junzhi Zhang: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
Jihong Dai: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
Huan Zhong: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
Wuying Chu: Department of Bioengneering and Environmental Science, Changsha University, Changsha, Hunan 410000, China
Wencheng Xu: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
Yi Hu: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China; Corresponding authors at: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China.
Kaijian Chen: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China; Corresponding authors at: Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China.

Journal volume & issue: Vol. 32
p. 101701

Abstract

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An eight-week feeding trial explored the mechanism of methionine supplementation (0 g/kg, 4 g/kg, 8 g/kg, 12 g/kg) in a high-fat diet (120 g/kg fat, HFD) on the growth performance and hepatic lipid metabolism of M. albus (initial weight 25.03 ± 0.13 g) based on a basic diet (60 g/kg fat), named as Con, HFD+M0, HFD+M4, HFD+M8, HFD+M12, respectively. 1) Compared with Con, high-fat diet decreased the survival rate and weight gain rate of M. albus, and significantly enhanced the feed conversion rate, hepatopancreas index and crude lipid content of M. albus (P < 0.05). Compared with HFD + M0, supplemented methionine markedly enhanced weight gain rate of M. albus (P < 0.05), and significantly decreased feed conversion rate and crude lipid content of M. albus (P < 0.05). 2) Compared with Con, hepatic lipoprotein lipase, fatty acid synthetase, triglyceride, cholesterol and fatty acid (C18:2n6, C20:5n3, SFA and MUFA) of HFD + M0 were markedly improved (P < 0.05); compared with HFD + M0, hepatic fatty acid synthase, triglyceride, cholesterol and fatty acid (C18:0, SFA and n-6PUFA) of HFD + M8 were significantly decreased (P < 0.05). Additionally, the proportion of hepatic vacuoles of HFD + M0 was higher than that in Con; compared with HFD + M0, hepatic cells of HFD + M8 were more regularly, and the proportion of hepatic vacuoles and lipid droplets decreased. 3) Compared with Con, hepatic srebf2, hsl, lpl, srebf1, cpt1 and cpt2 were remarkably up-regulated in HFD+M0 (P < 0.05); compared with HFD+M0, hepatic gcn2, eif2a, srebf2, srebf1 and fas were remarkably down-regulated in HFD+M8 (P < 0.05). Additionally, compared with HFD + M0, SREBP1 protein expression of HFD + M8 were markedly down-regulated (P < 0.01). In conclusion, dietary methionine mainly reduced hepatic lipid abnormal deposition by reducing hepatic fatty acid synthesis.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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