Osmanthus-Loaded PVP/PVA Hydrogel Inhibits the Proliferation and Migration of Oral Squamous Cell Carcinoma Cells CAL-27

Bin Huang; Chizhou Wu; Yuzhu Hu; Lu Rao; Mingzhe Yang; Mengyao Zhao; Huangqin Chen; Yuesheng Li

doi:10.3390/polym14245399

Polymers (Dec 2022)

Osmanthus-Loaded PVP/PVA Hydrogel Inhibits the Proliferation and Migration of Oral Squamous Cell Carcinoma Cells CAL-27

Bin Huang,
Chizhou Wu,
Yuzhu Hu,
Lu Rao,
Mingzhe Yang,
Mengyao Zhao,
Huangqin Chen,
Yuesheng Li

Affiliations

Bin Huang: Department of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China
Chizhou Wu: Department of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China
Yuzhu Hu: Department of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China
Lu Rao: Hubei Key Laboratory of Radiation Chemistry and Functional Materials, Non-Power Nuclear Technology Collaborative Innovation Center, Hubei University of Science and Technology, Xianning 437100, China
Mingzhe Yang: Department of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China
Mengyao Zhao: Department of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China
Huangqin Chen: Department of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China
Yuesheng Li: Hubei Key Laboratory of Radiation Chemistry and Functional Materials, Non-Power Nuclear Technology Collaborative Innovation Center, Hubei University of Science and Technology, Xianning 437100, China

DOI: https://doi.org/10.3390/polym14245399
Journal volume & issue: Vol. 14, no. 24
p. 5399

Abstract

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Conventional medical agents for oral squamous cell carcinoma (OSCC) with some adverse effects no longer meet the needs of the public. In this study, the prognosis-related hub genes of osmanthus-targeted therapy for OSCC were predicted and analyzed by network pharmacology and molecular docking. Osmanthus was extracted using the ethanol reflux method and osmanthus-loaded PVP/PVA (OF/PVP/PVA) hydrogel was prepared by electron beam radiation. The molecular structure, crystal structure and microscopic morphology of hydrogels were observed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM), respectively. OSCC cells CAL-27 were cultured with OF/PVP/PVA hydrogel at different concentrations of extract to discover cell proliferation by MTT assay. The scratching test and JC-1 staining were used to observe the migration and mitochondrial membrane potential. Through experimental exploration, we found that a total of six prognosis-related targets were predicted, which are PYGL, AURKA, SQLE, etc., and osmanthus extract had good binding activity to AURKA. In vitro, except for proliferation inhibition, OF/PVP/PVA hydrogel prevented cell migration and changed the mitochondrial membrane potential of CAL-27 cells at a concentration equal to or greater than 50 μg/mL (p p < 0.05).

Published in Polymers

ISSN: 2073-4360 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/polymers/

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