Infection and Drug Resistance (Jun 2022)
Emergence of blaOXA-1- and mph(A)-Producing Vibrio furnissii Isolated from Hospital Sewage
Abstract
Haishan Mo,1,* Xu Lin,1,* Ming Liu,2 Hui Liang1 1Department of General Surgery, Zhuhai People’s Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, People’s Republic of China; 2College of Oceanography, Hohai University, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hui Liang, Department of General Surgery, Zhuhai People’s Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, People’s Republic of China, Email [email protected]: Cephalosporins and azithromycin are clinical antibiotics used to treat infections. Co-resistance to cephalosporins and azithromycin has been observed in some Enterobacterales, but it has only rarely been reported in Vibrio species. In this study, we isolated a cephalosporin- and azithromycin-resistant V. furnissii strain, VFN3, from hospital sewage. Whole-genome sequencing results showed that the strain VFN3 possesses an IncA/C2 plasmid, pVFN3-blaOXA-193K. This conjugative plasmid carries several clinically relevant drug resistance genes, including mph(A) and blaOXA-1. We also found that in the strain VFN3, mph(A) and blaOXA-1 are surrounded by insertion sequences and class I integrons, respectively. These data suggest that mobile elements mediate the transfer of mph(A) and blaOXA-1. This is the first reported Vibrio species that possesses an mph(A)- and blaOXA-1-bearing conjugative plasmid. The emergence of this conjugative multi-drug-resistance plasmid is of great concern to public health.Keywords: mph(A), blaOXA-1, conjugative plasmid, Vibrio furnissii
