Baseline Sensitivity and Resistance of <i>Botrytis cinerea</i> to Penthiopyrad in Hebei Province, China
Qiuyan Bi,
Fen Lu,
Kexin Yang,
Jie Wu,
Shangqing Zhang,
Xiuying Han,
Wenqiao Wang,
Jianjiang Zhao
Affiliations
Qiuyan Bi
Key Laboratory of Integrated Pest Management on Crops in the Northern Region of North China, IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Ministry of Agriculture, Baoding 071000, China
Fen Lu
Key Laboratory of Integrated Pest Management on Crops in the Northern Region of North China, IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Ministry of Agriculture, Baoding 071000, China
Kexin Yang
Key Laboratory of Integrated Pest Management on Crops in the Northern Region of North China, IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Ministry of Agriculture, Baoding 071000, China
Jie Wu
Key Laboratory of Integrated Pest Management on Crops in the Northern Region of North China, IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Ministry of Agriculture, Baoding 071000, China
Shangqing Zhang
Tangshan Academy of Agricultural Sciences, Tangshan 063000, China
Xiuying Han
Key Laboratory of Integrated Pest Management on Crops in the Northern Region of North China, IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Ministry of Agriculture, Baoding 071000, China
Wenqiao Wang
Key Laboratory of Integrated Pest Management on Crops in the Northern Region of North China, IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Ministry of Agriculture, Baoding 071000, China
Jianjiang Zhao
Key Laboratory of Integrated Pest Management on Crops in the Northern Region of North China, IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Ministry of Agriculture, Baoding 071000, China
Tomato gray mold (TGM), caused by Botrytis cinerea, is a destructive disease of greenhouse tomatoes. Its control mainly depends on the application of chemical fungicides. In China, penthiopyrad has been registered for the management of TGM for about 3 years. In this study, the baseline sensitivity of B. cinerea to penthiopyrad was measured based on mycelial growth and spore germination, using 131 wild isolates collected from Hebei Province. The mean EC50 values 1.054 ± 0.633 mg/L (on mycelial growth) and 0.101 ± 0.037 mg/L (on conidia germination) were used as the baseline sensitivity. Isolates (187) of B. cinerea, sampled from eight regions in Hebei province during 2021 and 2022, were used for resistance studies by mycelial growth method. Based on the resistance factor, these isolates were considered: sensitive (=65 isolates), low (=39 isolates), moderate (=38 isolates), and high (=45 isolates) resistance. All 15 isolates from Raoyang were sensitive to penthiopyrad. Four mutation types in the sdhB subunit, namely H272R, N230I, P225F, and P225L, were associated with the resistance to penthiopyrad. A positive cross-resistance was also recorded between penthiopyrad and fluopyram, boscalid, or pydiflumetofen. These results provide useful information on the chemical control of TGM.
